双糖链蛋白聚糖对大肠癌细胞生长作用研究

邢晓静; 郑洪新; 宋纯

双糖链蛋白聚糖对大肠癌细胞生长作用研究

1.
辽宁省肿瘤医院消化肿瘤科辽宁中医药大学博士后在站,辽宁沈阳,110042;
2.
辽宁中医药大学,辽宁沈阳,110032;
3.
辽宁省肿瘤医院,辽宁沈阳,110042

基金项目: 辽宁省百千万人才项目资助项目，辽宁省自然科学基金资助项目

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中图分类号: R735.3
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- 发布日期: 2013-01-15

Effect of biglycan on growth of colon cancer cells

摘要

摘要: 目的研究Biglycan对大肠癌细胞HT-29和SW480侵袭、凋亡等生长作用影响.方法采用Transwell方法检测Biglycan对大肠癌细胞HT-29和SW480的侵袭能力影响;AV/PI染色检测细胞凋亡情况;Western blot法检测加药处理前后细胞内Rb、pRb和Ras表达变化.结果 100 nmol/L Biglycan和50 nmol/L Biglycan可抑制大肠癌细胞HT-29和SW480侵袭转移,同时可诱导HT-29、SW480细胞发生凋亡;经Biglycan处理后,细胞内Rb总蛋白无显著性变化,pRb和Ras出现下调趋势.结论 Biglycan可抑制大肠癌细胞HT-29和SW480侵袭转移、诱导细胞凋亡,下调pRb和Ras的表达、调控细胞周期从而抑制细胞生长为其可能的作用机制.
- 双糖链蛋白聚糖 /
- 大肠癌细胞 /
- 生长

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参考文献(12)

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双糖链蛋白聚糖对大肠癌细胞生长作用研究

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出版历程

Effect of biglycan on growth of colon cancer cells

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