抗纤复方对纤维肝大鼠肝细胞功能表达的影响

EFFECT OF ANTI-FIBROSIS COMPOND PRESCRIPTION ON PRIMARY CULTURE FIBROUS HEPATOCYTE FUNCTIONS

摘要: 目的 :探讨抗纤复方对体外培养纤维肝大鼠肝细胞功能表达的影响。方法 :常规培养纤维肝大鼠肝细胞 ,抗纤复方温育肝细胞 48h ,[3H] -TdR掺入法测定细胞增殖 ,[3H] -Pro掺入法测定细胞活力 ,用PAS法染色糖元 ,用偶氮偶联法染色碱性磷酸酶。结果 :抗纤复方药物血清的提高肝细胞活力的和促进肝糖元合成 ,抑制纤维肝细胞增殖与碱性磷酸酶活性的作用。结论 :抗纤复方具有调节肝细胞的异常代谢和功能状态。

Abstract: Objective:To study the effect of anti-fibrosis compond priscription (ACP) on primary culture fibrous hepatocyte functions such as proliferation,viability,glycogen and alkaline phosphorase. Mehtods:primary cultured fibrous hepatocyes were cultured wity the regular metyod, and incubated with 10 times drug sera of ACP for 48h. The cell proliferation was measured by[ 3H]-TdR incorporation, cell viablilty by [ 3H]Proline incorporation and cell glycogen by periodic acibschiff reaction.Results: The ACP could promote the viablilty and syhthetic glycogen of fibrous hepatocytes, inhibit the proliferation and ALP of fibrous hepatocytes, 10% ten-fold drug sera of ACP showed the best effect.Conclusion:The ACP could regulate the abnormal metabolism and function of hepatocytes.