Expression levels and significance of lipopolysaccharide binding protein in tissue, serum and serum exosomes of patients with oral squamous cell carcinoma
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摘要: 目的 分析口腔鳞状细胞癌(OSCC)患者血清、组织、血清外泌体中脂多糖结合蛋白(LBP)的表达及意义。 方法 选取确诊为OSCC的60例患者为观察组,同时选取60例健康体检者为对照组。比较OSCC患者癌组织、癌旁组织中LBP mRNA表达水平,分析OSCC患者癌组织中LBP mRNA的表达水平与临床特征的关系。检测健康体检者与OSCC患者血清外泌体以及血清LBP表达水平,并分析OSCC患者血清LBP表达量与临床特征的关系。统计并分析OSCC患者血清外泌体LBP表达量与临床特征的关系。 结果 吸烟、饮酒患者中LBP mRNA表达量在癌组织中较高,差异有统计学意义(P<0.05)。2组血清LBP表达水平比较,差异有统计学意义(P<0.05)。淋巴转移、饮酒的OSCC患者血清外泌体LBP mRNA表达量高于无淋巴转移及不饮酒患者,差异有统计学意义(P<0.05)。OSCC患者血清LBP mRNA表达量与TNM分期、淋巴转移、分化程度、吸烟、饮酒等临床特征无显著相关性(P>0.05)。 结论 LBP表达水平与OSCC疾病有一定相关性,能够作为诊断疾病的指标。Abstract: Objective To analyze the expression levels and significance of lipopolysaccharide binding protein(LBP)in serum, tissues and serum exosomes in oral squamous cell carcinoma(OSCC). Methods A total of 60 patients diagnosed as OSCC were selected as observation group, and 60 healthy people with physical examinations were selected as control group. The expression levels of LBP mRNA in cancer tissues and adjacent tissues of OSCC patients were compared, and the relationship between the expression levels of LBP mRNA in cancer tissues of OSCC patients and clinical characteristics was analyzed. Serum exosomes and serum LBP expression levels of healthy subjects and OSCC patients were detected, and the relationships between serum LBP expression level and clinical characteristics of OSCC patients as well as between serum exosome LBP expression and clinical characteristics of OSCC patients were analyzed. Results OSBP patients with smoking and alcohol drinking had significantly higher LBP mRNA expression levels(P<0.05); the levels of serum LBP expression of two groups showed significant differences(P<0.05). OSCC patients with lymphatic metastasis and alcohol drinking habits had higher levels of LBP expression in serum exosomes than those without(P<0.05). There were no significant correlations between serum LBP mRNA expression - and TNM stage, lymphatic metastasis, differentiation degree, smoking, drinking and other clinical characteristics(P>0.05). Conclusion The expression level of LBP is correlated with OSCC disease, which can be used as a indicator in diagnosing the disease.
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