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白藜芦醇对绝经后骨质疏松症大鼠的促细胞增殖作用

邹庆峰, 李守民, 舒隆钧, 刘世昌, 张继源

邹庆峰, 李守民, 舒隆钧, 刘世昌, 张继源. 白藜芦醇对绝经后骨质疏松症大鼠的促细胞增殖作用[J]. 实用临床医药杂志, 2020, 24(19): 86-89. DOI: 10.7619/jcmp.202019025
引用本文: 邹庆峰, 李守民, 舒隆钧, 刘世昌, 张继源. 白藜芦醇对绝经后骨质疏松症大鼠的促细胞增殖作用[J]. 实用临床医药杂志, 2020, 24(19): 86-89. DOI: 10.7619/jcmp.202019025
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ZOU Qingfeng, LI Shoumin, SHU Longjun, LIU Shichang, ZHANG Jiyuan. Role of resveratrol in promoting cell proliferation of rats with ovariectomized osteoporosis[J]. Journal of Clinical Medicine in Practice, 2020, 24(19): 86-89. DOI: 10.7619/jcmp.202019025
Citation: ZOU Qingfeng, LI Shoumin, SHU Longjun, LIU Shichang, ZHANG Jiyuan. Role of resveratrol in promoting cell proliferation of rats with ovariectomized osteoporosis[J]. Journal of Clinical Medicine in Practice, 2020, 24(19): 86-89. DOI: 10.7619/jcmp.202019025
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白藜芦醇对绝经后骨质疏松症大鼠的促细胞增殖作用

  • 云南省大理市第一人民医院 骨科, 云南 大理, 671000

基金项目: 

云南省科技厅-昆明医科大学联合基金资助项目(2014FB006)

云南省大理市科技局基金资助项目(2016KJ35)

详细信息
    通讯作者:

    张继源,E-mail:zhangjy@163.com

  • 中图分类号: R714.257

Role of resveratrol in promoting cell proliferation of rats with ovariectomized osteoporosis

  • Department of Orthopedics, Dali First People's Hospital in Yunnan Province, Dali, Yunnan, 671000

摘要

    摘要
  • 摘要: 目的 构建绝经后骨质疏松症(PMOP)大鼠模型,探讨白藜芦醇(RES)在PMOP中的促细胞增殖作用。 方法 将80只清洁级SD大鼠随机分为对照组、PMOP组、RES低剂量组(60 mg/kg)、RES中剂量组(80 mg/kg)、RES高剂量组(100 mg/kg), 每组16只。大鼠行双侧卵巢摘除术,术后1周给予RES预防性治疗,每月称重体质量。24周后心脏采血处死,取双侧股骨,采用骨密度仪测量骨密度变化,通过逆转录聚合酶链式反应(RT-PCR)检测骨组织中p53和Cyclin-D1水平的变化, Western blot检测各组大鼠Notch-1蛋白表达情况。 结果 PMOP组大鼠股骨和椎骨的骨密度降低, RES不同剂量组大鼠股骨和椎骨的骨密度较 PMOP组升高,差异均有统计学意义(P<0.05)。与对照组相比, PMOP组p53和Cyclin-D1的mRNA转录水平显著增高, RES不同剂量组的Cyclin-D1的mRNA转录水平较PMOP组升高,而p53的mRNA转录水平降低,差异均有统计学意义(P<0.05)。Western blot结果显示, PMOP组的Notch-1表达水平显著抑制, RES不同剂量组Notch-1蛋白表达较PMOP组升高,差异有统计学意义(P<0.05)。 结论 RES调控Notch-1信号通路来促进骨组织中的细胞增殖,发挥拮抗骨质疏松症的作用。
    Abstract: Objective To establish a rat model of postmenopausal osteoporosis(PMOP)and explore role of resveratrol(RES)in promoting cell proliferation of rats with PMOP. Methods Totally 80 clean SD rats were randomly divided into control group, PMOP group, low dose RES group(60 mg/kg), medium dose RES group(80 mg/kg)and high dose RES group(100 mg/kg), with 16 rats in each group. Rats were treated with bilateral ovariectomy and RES as preventive treatment one week after operation, and body weight was measured every month. After 24 weeks, blood samples were collected from the heart after sacrifice and bilateral femurs were taken. Bone mineral density was measured by bone densitometer, levels of p53 and Cyclin-D1 were detected by reverse transcription polymerase chain reaction(RT-PCR), and the expression of Notch-1 protein was detected by Western blot. Results The bone mineral densities of femur and vertebrae in the PMOP group decreased significantly, and bone mineral densities of femur and vertebrae in the groups with different RES concentrations were significantly higher than those in the PMOP group(P<0.05). Compared with the control group, the mRNA transcription levels of p53 and Cyclin-D1 in the PMOP group were significantly higher, and the mRNA transcription levels of Cyclin-D1 in the groups with different RES concentrations were significantly higher than that in the PMOP group, while the mRNA transcription level of p53 was significantly lower(P<0.05). Western blot results showed that the expression level of Notch-1 in the PMOP group was significantly inhibited, and the expression level of Notch-1 in the groups with different RES concentrations was significantly higher than that in the PMOP group(P<0.05). Conclusion RES plays a role of antagonizing osteoporosis through regulating Notch-1 signaling pathway for promotion of cell proliferation in bone tissue.
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  • 收稿日期:  2020-08-19

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