酶联免疫吸附测定与免疫印迹试验检测人类免疫缺陷病毒抗体的差异性分析

杜萍萍; 李芳

doi:10.7619/jcmp.202013004

酶联免疫吸附测定与免疫印迹试验检测人类免疫缺陷病毒抗体的差异性分析

杜萍萍¹,
李芳^2, ,

1. 陕西省铜川市人民医院检验科, 陕西铜川, 727000;
2. 中国人民警察武装部队陕西省总队医院检验与病理科, 陕西西安, 710054

详细信息

通讯作者:
李芳,E-mail:nuts521@163.com

中图分类号: R392.11
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出版历程
- 收稿日期: 2020-03-24

Differential analysis of human immunodeficiency virus-related antibody detected by enzyme linked immunosorbent assay and western blot

DU Pingping¹,
LI Fang^2, ,

1. Department of Laboratory Medicine, Tongchuan City People's Hospital, Tongchuan, Shaanxi, 727000;
2. Department of Laboratory and Pathology, Shaanxi Provincial General Corps Hospital of Chinese People's Police Armed Forces, Xi'an, Shaanxi, 710054

摘要

摘要: 目的探讨酶联免疫吸附测定(ELISA)与免疫印迹试验(WB)在人类免疫缺陷病毒(HIV)抗体检测中的差异性。方法选取陕西省铜川地区进行HIV抗体检测的疑似HIV感染患者105例为研究对象,初筛后进行ELISA、反转录聚合酶链反应(RT-PCR)复核检测后再经WB验证。分析初筛后ELISA、RT-PCR复核检测结果与WB验证结果的差异性。比较ELISA检测得出的质控物吸光度值与临界值的比值(S/CO)与WB验证结果的差异,并观察WB反应带型结果。结果 105例疑似HIV感染患者ELISA、RT-PCR复核检测结果显示,双阳性98例,WB验证后显示阳性87例,符合率为88.78%(87/98)。ELISA检测结果为阳性但RT-PCR检测为阴性的样本经WB验证后确定阳性1例,符合率为20.00%(1/5)。ELISA检测得出的S/CO值<1及S/CO值≥10时与WB验证结果的符合率最高。WB证实为阳性的87例样本中,gp160、gp120出现率均为100.00%,gp41、p24、p17出现率分别为95.40%(83/87)、64.37%(56/87)、56.32%(49/87), 另有2.30%(2/87)显示无HIV抗体特异带。结论 ELISA、WB用于HIV抗体检测均具有一定价值,对复核检测结果采用WB验证可提高诊断符合率,但WB验证后的样本中亦存在阴性或不确定性样本,因此应对此类患者进行重点追踪并实施进一步检测。
- 酶联免疫吸附测定 /
- 免疫印迹试验 /
- 人类免疫缺陷病毒 /
- 复核检测 /
- 验证结果 /
- 抗体特异带 /
- 符合率
Abstract: Objective To investigate the difference of enzyme linked immunosorbent assay(ELISA)and western blotting(WB)in detection of human immunodeficiency virus(HIV)antibody. Methods HIV antibody detections were performed for 105 suspected HIV-infected patients in areas of Tongchuan City of Shaanxi Province, after the initial screening by ELISA and recheck of reverse transcription-polymerase chain reaction(RT-PCR), the results were re-verified by WB. The differences between result of early screening by detection of ELISA as well as recheck of RT-PCR and WB verification, and between ratio of the absorbance value to cut-off value(S/CO)of ELISA detection and results of WB verification were analyzed. Result of reaction band type of WB test was observed. Results Out of 105 suspected HIV-infected patients, 98 cases were diagnosed as double positive findings detected by ELISA and RT-PCR, and 87 positive cases were confirmed as HIV infection by WB, with a coincidence rate of 88.78%(87/98). In patients with positive results by ELISA but negative results by RT-PCR, one case was confirmed as positive result by WB, with a coincidence rate of 20.00%(1/5). The coincidence rates were the highest when S/CO value<1 and S/CO≥10 obtained by ELISA test compared with the WB verification result. Among the 87 positive samples confirmed by WB, the occurrence rates of gp160 and gp120 reached 100.00%, followed by gp41, p24 - and p17, with their occurrence rates of 95.40, 64.37% and 56.32%, respectively, and another 2.30% samples showed no specific HIV antibody band. Conclusion Both ELISA and WB detection can improve the coincidence rate of HIV antibody detection. However, there are negative or uncertain samples after WB validation. Therefore, such patients should be tracked and given further testing.
- enzyme-linked immunosorbent assay /
- western blotting /
- human immunodeficiency virus /
- recheck test /
- verification results /
- antibody specific band /
- coincidence rate

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参考文献(17)

肖晓华, 吴金玲, 廖惠, 等. 基于结构方程模型的大学生HIV检测知识知晓及检测意向分析[J]. 中国艾滋病性病, 2019, 25(12): 1250-1253.

陈旭富, 游佳玲, 吕磊. 病毒载量试验与抗体补充试验对HIV抗体不确定样本诊断效果对比研究[J]. 中国艾滋病性病, 2019, 25(12): 1222-1224.

赫晓霞, 程焕义, 王月华, 等. 干血斑用于HIV抗体确证(重组免疫印迹法)方法建立与初步应用[J]. 中国艾滋病性病, 2019, 25(11): 1097-1100.

张念, 张米, 李健健, 等. 33例HIV-1抗体免疫印迹试验不确定结果分析[J]. 热带医学杂志, 2019, 19(10): 1217-1220

, 1253.

崔圣敏. 天津市河北区HIV抗体初筛阳性样本的复检及免疫印迹试验结果分析[J]. 预防医学论坛, 2019, 25(7): 529-531

, 535.

张月娟, 倪争鸣, 王娜, 等. 艾滋病筛检人群HIV-1抗体确证阳性率分析[J]. 上海预防医学, 2019, 31(6): 514-519.

袁丹, 谭敏, 金丹, 等. 使用HIV初筛方法定性判断的可行性研究[J]. 预防医学情报杂志, 2019, 35(3): 228-230.

刘洋, 杨慧娣, 蒋苏, 等. 胶体层析法与酶联免疫吸附试验在急诊艾滋病病毒(HIV)抗体初次筛查中的比较分析[J]. 中国性科学, 2019, 28(1): 118-121.

周昀, 杨新智, 周秋颖, 等. HIV抗体初筛阳性标本的复检与确证结果分析[J]. 沈阳医学院学报, 2019, 21(01): 56-58

, 61.

张荣秋, 李莉, 张建梅, 等. 2013-2017年厦门市HIV抗体蛋白印迹实验检测结果分析[J]. 现代预防医学, 2018, 45(21): 3981-3984.[11] 李艳, 潘彤, 李浩龙, 等. 液相芯片法验证蛋白质免疫印迹试验检测HIV-1结果的敏感性和特异性[J]. 实用检验医师杂志, 2018, 10(3): 176-178

, 181.

杨育红, 陈杰毅, 占凌云. HIV抗体ELISA和胶体硒联合检测与免疫印迹试验的结果对比分析[J]. 实验与检验医学, 2018, 36(2): 273-275.

董雪, 刘建敏, 李福安, 等. 全自动免疫印迹检测系统在HIV抗体确证试验中的应用研究[J]. 中国卫生检验杂志, 2018, 28(5): 537-539.

胡二红. 三种HIV抗体初筛法与确证法检测结果的对比分析[J]. 内蒙古医学杂志, 2017, 49(7): 864-865.

覃彦琳, 叶瑞国, 宁珺茹. 514份HIV抗体初筛阳性标本复核与确证检测结果对比分析[J]. 应用预防医学, 2017, 23(03): 227-228

, 231.

董文斌, 蔡英, 赵金仙, 等. HIV抗体筛查阳性标本中确证阴性比例及相关因素分析[J]. 现代预防医学, 2017, 44(10): 1879-1882

, 1886.

杨芬兰, 穆廷杰, 陈涛. 抗-HIV抗体筛查与免疫印迹试验两种检测方法的对比研究[J]. 国际检验医学杂志, 2017, 38(7): 948-950.

张庆娟, 张志坤, 毛立超, 等. 唐山市HIV抗体确证试验的替代策略研究[J]. 中国艾滋病性病, 2017, 23(2): 98-101.

施引文献(8)

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2.	廖双华，郑文武，刘盼，黄冬梅，刘茜，李丹. 579例急性心肌梗死患者临床特点分析. 西南军医. 2018(01): 64-67 . 百度学术
3.	解学荣. 糖尿病合并急性心肌梗死患者临床特点分析. 糖尿病新世界. 2018(09): 33-34 . 百度学术
4.	周升，黄名权，张鹏，陈德方. 糖尿病患者的心率减速力和连续心率减速力研究. 海南医学. 2018(24): 3436-3439 . 百度学术
5.	翁菲菲，冯文化. 血管过度负荷指数检测对2型糖尿病合并急性心肌梗死患者预后临床意义的观察. 中国糖尿病杂志. 2018(12): 988-992 . 百度学术