维生素B<sub>6</sub>修饰四氧化三铁纳米酶活性及其抗变异链球菌作用

成荣; 王萧; 程璐; 张唯; 丁黎明; 高利增; 许卓斌

doi:10.7619/jcmp.202008001

维生素B₆修饰四氧化三铁纳米酶活性及其抗变异链球菌作用

成荣¹,
王萧¹,
程璐¹,
张唯¹,
丁黎明¹,
高利增^1,2,,
许卓斌^1,2,

扬州大学, 1. 医学院;
2. 转化医学研究院;
江苏扬州, 225001

基金项目:

江苏省大学生创新创业训练计划项目(201711117036Z)

国家自然科学基金资助项目(81901879)

扬州大学大学生学术科技创新基金项目(X20190727)

江苏省大学生创新创业训练计划项目(201811117110E)

详细信息

通讯作者:
高利增,E-mail:lzgao@yzu.edu.cn

许卓斌,E-mail:xuzb@yzu.edu.cn

中图分类号: R781.5
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出版历程
- 收稿日期: 2020-02-09

Activity of Fe₃O₄ nanozyme with vitamin B₆ modification and its anti-Streptococcus mutans effect

1. Medical College;
2. Institute of Translational Medicine, Yangzhou University, Yangzhou, Jiangsu, 225001

摘要

摘要: 目的探讨维生素B₆修饰对四氧化三铁纳米酶(IONzymes)活性的影响以及对口腔变异链球菌(S. mutans)的抑制作用。方法通过溶剂热法合成IONzymes并进行维生素B₆修饰,观察经维生素B₆修饰的四氧化三铁纳米酶(VB₆-IONzymes)形貌的电镜表征,分析其催化活性的改变。结果与未经修饰的IONzymes相比, VB₆-IONzymes体积大幅减小,过氧化物酶活性显著增强。VB₆-IONzymes具有显著的抗口腔S. mutans的作用(P<0.001), 且能显著促进1%过氧化氢(H₂O₂)对S. mutans形成生物膜的抑制作用(P<0.001)。讨论维生素B₆修饰不仅可以提高IONzymes的过氧化物酶与过氧化氢酶活性,还在抗口腔龋齿方面具有潜在的应用价值。
- 纳米酶 /
- 四氧化三铁 /
- 变异链球菌 /
- 催化 /
- 生物膜
Abstract: Objective To investigate the influence of vitamin B₆ modification on enzymatic activity of ferric iron oxide nanozymes(IONzymes)and its inhibitory effect on oral Streptococcus mutans(S. mutans). Methods IONzymes was modified with vitamin B₆ by a solvothermal reaction. The morphology of IONzymes with vitamin B₆ modification(VB₆-IONzymes)was observed by electron microscope, and change of its catalytic activity was analyzed. Results Compared with the unmodified IONzymes, the volume of VB₆-IONzymes was greatly reduced and its peroxidase activity was significanly enhanced. Bacteriostatic experiment showed that VB₆-IONzymes had a significant ability to inhibit S. mutans, and can significantly promote the inhibitory effect of 1% H₂O₂ on the S. mutans biofilm formation. Conclusion Vitamin B₆ modification not only improves the enzymatic activity of IONzymes, but also shows potential application value in preventing dental caries.
- nano-enzyme /
- ferric iron oxide /
- Streptococcus mutans /
- catalysis /
- biofilm

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