Expression of lysosomal associated membrane protein 2a in tissue of esophageal cancer and its clinical significance
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摘要: 目的 探讨溶酶体相关膜蛋白2a(LAMP2a)在食管癌组织中的表达及其临床意义。 方法 采用免疫组织化学方法检测58例患者的食管癌组织标本和30例正常食管组织中LAMP2a的表达水平,分析其与临床病理特征的相关性。 结果 LAMP2a在食管癌组织中呈显著高表达,与肿瘤临床分期(P<0.001)、T分期(P=0.043)及分化程度(P=0.018)密切相关,与性别(P=0.740)、年龄(P=0.590)和淋巴结转移(P=0.188)无显著相关性; LAMP2a表达水平与患者的预后呈负相关(P<0.05); Cox比例风险模型显示,肿瘤浸润深度和LAMP2a蛋白表达是食管癌患者独立影响因素(P<0.05)。 结论 LAMP2a的表达可成为肿瘤早期诊断及预后状况评估的有效指标。
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关键词:
- 溶酶体相关膜蛋白2a /
- 食管癌 /
- 免疫组化法 /
- 预后
Abstract: Objective To investigate the expression of lysosomal associated membrane protein 2a(LAMP2a)in tissue of esophageal cancer and its clinical significance. Methods The expressions of LAMP2a in 58 specimens of esophageal cancer and 30 specimens of normal esophageal tissues were detected by immunohistochemical methods, and its correlation with clinicopathological features was analyzed. Results The expression of LAMP2a was significantly higher in esophageal cancer tissues, which was closely correlated to tumor clinical staging(P<0.001), T staging(P=0.043)and differentiation degree(P=0.018), but no correlation with gender(P=0.740), age(P=0.590)and lymph node metastasis(P=0.188); the expression of LAMP2a was negatively correlated with the prognosis of patients(P<0.05). Cox proportional hazard model showed that depth of invasion and expression of LAMP2a protein were independent risk factors for esophageal cancer(P<0.05). Conclusion The expression of LAMP2a can be used as an effective indicator in early diagnosis and prognosis evaluation of tumors. -
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陈永宏, 张沛刚, 王耀勇, 等. MTLC在食管癌中的表达及意义[J]. 基层医学论坛, 2015, 19(4): 433-434. 刘敏, 郭晓娟, 张红霞, 等. 表皮生长因子受体及Ⅲ型突变体在食管癌的表达及意义[J]. 中国癌症杂志, 2009, 19(10): 729-734. 谭遥, 卢喜, 吴万艳, 等. 食管癌细胞中Let-7miRNA表达对CD8+ T影响分析[J]. 实用癌症杂志, 2019, 34(2): 178-182 , 186.
Endo Y, Furuta A, Nishino I. Danon disease: a phenotypic expression of LAMP-2 deficiency[J]. Acta Neuropathol, 2015, 129(3): 391-398.
Cuervo A M, Dice J F. Unique properties of lamp2a compared to other lamp2 isoforms[J]. J Cell Sci, 2000, 113(Pt 24): 4441-4450.
Sjödin S, Öhrfelt A, Brinkmalm G, et al. Targeting LAMP2 in human cerebrospinal fluid with a combination of immunopurification and high resolution parallel reaction monitoring mass spectrometry[J]. Clin Proteomics, 2016, 13: 4-8.
Wang R B, Liu Y T, Liu L, et al. Tumor cells induce LAMP2a expression in tumor-associated macrophage for cancer progression[J]. EBioMedicine, 2019, 40: 118-134.
马杰, 李宝生, 闫婧, 等. 食管癌组织中E-cadherin的表达及其与临床病理特征和预后关系的研究[J]. 中华肿瘤防治杂志, 2008, 15(1): 31-34. 田锋, 王天佑, 常栋, 等. 食管癌组织芯片COX-2和survivin表达及其与预后的关系[J]. 肿瘤防治研究, 2009, 36(3): 203-206. Eskelinen E L. Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy[J]. Mol Aspects Med, 2006, 27(5/6): 495-502.
Ding Z B, Fu X T, Shi Y H, et al. Lamp2a is required for tumor growth and promotes tumor recurrence of hepatocellular carcinoma[J]. Int J Oncol, 2016, 49(6): 2367-2376.
Lee K H, Lee J, Woo J, et al. Proteasome inhibitor-induced IκB/NF-κB activation is mediated by Nrf2-dependent light chain 3B induction in lung cancer cells[J]. Mol Cells, 2018, 41(12): 1008-1015.
Kurt R, Chandra P K, Aboulnasr F, et al. Chaperone-mediated autophagy targets IFNAR1 for lysosomal degradation in free fatty acid treated HCV cell culture[J]. PLoS One, 2015, 10(5): e0125962.
Arita S, Kinoshita Y, Ushida K, et al. High-fat diet feeding promotes stemness and precancerous changes in murine gastric mucosa mediated by leptin receptor signaling pathway[J]. Arch Biochem Biophys, 2016, 610: 16-24.
Giatromanolaki A, Sivridis E, Kalamida D, et al. Transcription factor EB expression in early breast cancer relates to lysosomal/autophagosomal markers and prognosis[J]. Clin Breast Cancer, 2017, 17(3): e119-e125.
Kalamida D, Giatromanolaki A, Koukourakis M I. Characterization of the “autophagic flux” in prostate cancer tissue biopsies by LC3A/LAMP2a immunofluorescence and confocal microscopy[J]. Methods Mol Biol, 2019, 1880: 555-560.
Kon M, Kiffin R, Koga H, et al. Chaperone-mediated autophagy is required for tumor growth[J]. Sci Transl Med, 2011, 3(109): 109ra117.
蔡维乐, 王璐, 周霞, 等. LAMP2抑制肝癌细胞增殖的机制[J]. 现代肿瘤医学, 2017, 25(9): 1365-1369. Zhou J F, Yang J J, Fan X, et al. Chaperone-mediated autophagy regulates proliferation by targeting RND3 in gastric cancer[J]. Autophagy, 2016, 12(3): 515-528.
Han Q, Deng Y C, Chen S, et al. Downregulation of ATG5-dependent macroautophagy by chaperone-mediated autophagy promotes breast cancer cell metastasis[J]. Sci Rep, 2017, 7(1): 4759.
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