Role of Actinidia Chinensis Planch root extract in radiosensitization for hepatoma cell Bel-7402
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摘要: 目的 探讨中华猕猴桃根提取物对肝癌Bel-7402细胞的放疗增敏作用。 方法 采用流式细胞仪分析中华猕猴桃根提取物对Bel-7402细胞凋亡及形态的影响。采用克隆形成法检测Bel-7402细胞存活分数,并绘制细胞存活曲线。 结果 流式细胞仪结果显示,与单纯放疗组、单纯药物组相比,联合放疗组Bel-7402凋亡细胞的比例显著增多。从凋亡细胞分布情况进行分析,药物主要引起中、晚期Bel-7402细胞凋亡和坏死,对早期细胞凋亡无明显影响。与单纯放疗组相比,联合放疗组正常细胞数量逐渐减少,而凋亡细胞数量逐渐增多。与单纯放疗组相比,联合放疗组细胞存活曲线显示细胞存活分数明显降低,表明中华猕猴桃根提取物对肝癌细胞Bel-7402具有放疗增敏作用。 结论 中华猕猴桃根提取物可能通过诱导细胞凋亡来对Bel-7402细胞产生放疗增敏作用,其机制可能是阻滞肿瘤细胞在G1/M期,从而引起DNA损伤。Abstract: Objective To explore the role of Actinidia Chinensis Planch root extract in radiosensitization for hepatoma cell Bel-7402. Methods Flow cytometry was used to analyze the effect of Actinidia Chinensis Planch root extract on apoptosis and morphology of hepatoma cell line Bel-7402. The cell survival fraction of Bel-7402 was measured by colony-forming method, and the cell survival curve was drawn. Results Flow cytometry showed that the proportion of apoptotic cells of Bel-7402 in the combined radiotherapy group was significantly higher than that in the radiotherapy group and the drug group. According to the analysis of the distribution of apoptotic cells, the drug mainly caused the apoptosis and necrosis of Bel-7402 cells in the middle and late stage, but had no significant effect on apoptosis of cells in early stage. Compared with the radiotherapy group, the number of normal cells in the combined radiotherapy group decreased gradually, while the number of apoptotic cells increased gradually. Compared with the radiotherapy group, the cell survival curve of the combined radiotherapy group showed that the cell survival fraction was significantly reduced, which indicated that the extract of Actinidia Chinensis Planch root had the radiosensitization effect on Bel-7402 cells. Conclusion Actinidia Chinensis Planch root extract may produce radiosensitization effect on Bel-7402 cells by inducing apoptosis of cells, and its mechanism may be involved in blocking tumor cells in G1/M phase and cause DNA damage.
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