Research on role of miR-144/451 in regulation of transferrin receptor 1
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摘要: 目的 探讨miR-144/451对红细胞转铁蛋白受体1(Tfr1)的调控作用。 方法 流式细胞术检测野生型小鼠(WT)和miR-144/451基因敲除小鼠(mKO)的胚胎肝、骨髓、脾脏及外周血红细胞中Tfr1的平均荧光强度(MFI)。实时定量PCR(qRT-PCR)检测骨髓和脾脏有核红细胞中Tfr1 mRNA的表达水平。 结果 与WT小鼠比较, mKO小鼠胚胎肝、骨髓、脾脏及外周血红细胞中Tfr1的MFI显著降低(P<0.05或P<0.01)。mKO小鼠骨髓及脾脏的有核红细胞中Tfr1 mRNA的表达水平显著高于WT小鼠(P<0.05)。 结论 miR-144/451参与调控红细胞中的Tfr1, 从而影响红细胞的生成。本研究为探讨mKO小鼠贫血的原因指明了方向。Abstract: Objective To explore the role of miR-144/451 in regulation of transferrin receptor 1(Tfr1). Methods Mean fluorescence intensity(MFI)of Tfr1 of cells from fetal liver, bone marrow, spleen, and peripheral blood of wild-type mice(WT)and miR-144/451 knockout mice(mKO)was detected by flow cytometry. Quantitative real time PCR(qRT-PCR)was used to detect mRNA levels of Tfr1 in nucleated erythroid cells in bone marrow and spleen. Results Compared with WT mice, the MFI of Tfr1 of erythroid cells of fetal liver, bone marrow, spleen, and peripheral blood in mKO mice was significantly decreased(P<0.05 or P<0.01). The expression of Tfr1 mRNA increased in nucleated erythroid cells in bone marrow and spleen of mKO mice compared to WT mice. Conclusion miR-144/451 is involved in the regulation of Tfr1 in red cells, which may affect erythropoiesis. This article leads a direction for anemia reasons of mKO rats.
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Keywords:
- miR-144/451 /
- transferrin receptor 1 /
- erythrocytes /
- fluorescence intensity /
- bone marrow /
- regulation
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