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非整倍体结肠癌对顺铂的耐药性研究

张汉卿, 刘颖, 房晓

张汉卿, 刘颖, 房晓. 非整倍体结肠癌对顺铂的耐药性研究[J]. 实用临床医药杂志, 2020, 24(1): 42-45. DOI: 10.7619/jcmp.202001011
引用本文: 张汉卿, 刘颖, 房晓. 非整倍体结肠癌对顺铂的耐药性研究[J]. 实用临床医药杂志, 2020, 24(1): 42-45. DOI: 10.7619/jcmp.202001011
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ZHANG Hanqing, LIU Ying, FANG Xiao. Research on drug resistance of aneuploid colon cancer cells to cisplatin[J]. Journal of Clinical Medicine in Practice, 2020, 24(1): 42-45. DOI: 10.7619/jcmp.202001011
Citation: ZHANG Hanqing, LIU Ying, FANG Xiao. Research on drug resistance of aneuploid colon cancer cells to cisplatin[J]. Journal of Clinical Medicine in Practice, 2020, 24(1): 42-45. DOI: 10.7619/jcmp.202001011
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非整倍体结肠癌对顺铂的耐药性研究

  • 1. 扬州大学医学院, 江苏 扬州, 225001;

  • 2. 扬州大学临床医学院, 江苏 扬州, 225001

基金项目: 

江苏省青年医学重点人才培养项目(QNRC2016322)

国家自然科学基金资助项目(81402482)

详细信息
    通讯作者:

    房晓, E-mail: fangxiao@yzu. edu.cn

  • 中图分类号: R735.3

Research on drug resistance of aneuploid colon cancer cells to cisplatin

  • 1. Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001;

  • 2. Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu, 225001

摘要

    摘要
  • 摘要: 目的 探讨非整倍体结肠癌对顺铂的耐药性。 方法 结肠癌细胞HCT116经多西环素(Dox)诱导为敲低MAD2表达的为Dox(+)组(非整倍体),未经Dox处理的为Dox(-)组(整倍体)。顺铂处理2组细胞48 h, 以CCK-8法和细胞计数法分析顺铂对细胞生长的影响。Western blot法检测顺铂对细胞造成的凋亡影响。实时荧光定量即时聚合酶链锁反应(qRT-PCR)实验检测细胞中BAX、PUMA和NOXA凋亡基因表达情况。 结果 以不同浓度顺铂处理细胞,通过CCK-8实验和细胞计数实验检测发现, Dox(+)组细胞较Dox(-)组细胞对顺铂具有耐药性。Western Blot实验发现, Dox(+)组细胞的耐药性与其经顺铂处理后凋亡蛋白表达较低有关。qRT-PCR实验验证顺铂处理后Dox(+)组细胞较Dox(-)组细胞凋亡基因PUMA、NOXA、BAX表达显著减少(P<0.05或P<0.01)。 结论 非整倍体结肠癌细胞对顺铂具有耐药性。
    Abstract: Objective To explore drug resistance of aneuploid colon cancer cells to cisplatin. Methods The HCT116 cells with expression of knockdown MAD2 induced by doxycycline(DOX)were designed as DOX(+)group(aneuploid), and HCT116 cells without process of DOX were designed as DOX(-)group(euploid). CCK-8 method was used to process cells in both groups, and CCK-8 test and cytometry were used to analyze the effect of cisplatin on cell growth. Quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the expression of pro-apoptotic genes BAX, PUMA and NOXA in the cells. Results Cells were treated with cisplatin at different concentrations. CCK-8 test and cytometry showed that cells in DOX(+)group were more resistant to cisplatin than those in DOX(-)group. Western blot showed that the resistance of cells in DOX(+)group was related to the lower expression of apoptotic protein after cisplatin treatment. The qRT-PCR showed that the expressions of apoptosis gene PUMA, NOXA and BAX in DOX(+)group were significantly lower than those in DOX(-)group(P<0.05 or P<0.01)after process of cisplatin. Conclusion The aneuploid colon cancer cells are resistant to cisplatin treatment.
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出版历程
  • 收稿日期:  2019-10-19
  • 网络出版日期:  2020-12-22
  • 发布日期:  2020-08-06

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