Value of detection of mitogen-activated protein kinase 4 combined with vimentin in early diagnosis of endometrial cancer
-
摘要:目的 研究丝裂原活化蛋白激酶4(MAP2K4)和波形蛋白联合检测运用于子宫内膜癌中的诊断价值。方法 选择103例子宫内膜癌患者为研究对象, 检测MAP2K4、波形蛋白，探讨联合检测的诊断价值。结果 MAP2K4检测阳性率为37.86%(39/103), 低于波形蛋白的阳性率65.05%(67/103), 且阳性细胞所占比例积分及着色强度评分较波形蛋白更低，差异有统计学意义(P < 0.05); MAP2K4的阳性表达与患者的FIGO分期、淋巴转移有显著相关性，而波形蛋白的阳性表达与患者的FIGO分期、组织学分型、浸润深度及淋巴转移有显著相关性(P < 0.05)。MAP2K4和波形蛋白联合检测的灵敏度、特异性显著高于单一检测(P < 0.05)。结论 MAP2K4和波形蛋白联合检测运用于子宫内膜癌早期诊断中效果明显，为预后提供参考依据。
-
关键词:
- 波形蛋白 /
- 子宫内膜癌 /
- 丝裂原活化蛋白激酶4 /
- 诊断价值
Abstract:Objective To study the diagnostic value of combined detection of mitogen-activated protein kinase 4(MAP2K4) and vimentin in diagnosis of endometrial cancer.Methods A total of 103 patients with endometrial cancer were selected as study subjects. MAP2K4 and vimentin were detected respectively, and diagnostic value of combined detection was explored.Results The endometrial cancer-positive rate detected by MAP2K4 was significantly lower than that of vimentin[37.86% (39/103) vs. 65.05%(67/103)], the proportion score and staining intensity score of positive cells were also significantly lower than those of vimentin(P < 0.05). The positive expression of MAP2K4 was significantly correlated with FIGO staging and lymph node metastasis, while the positive expression of vimentin was significantly correlated with FIGO staging, histological types, invasion depth and lymph node metastasis (P < 0.05). The sensitivity and specificity of combined detection of MAP2K4 and vimentin were significantly higher than that of single detection(P < 0.05).Conclusion The combined detection of MAP2K4 and vimentin has obvious effect in the diagnosis of endometrial cancer, which can provide reference for prognosis. -
-
表 1 MAP2K4与波形蛋白阳性细胞所占比例积分及着色强度评分对比(x±s)
分 检测方式 n 阳性细胞所占比例积分 着色强度评分 MAP2K4 103 2.58±0.46 2.20±0.33 波形蛋白 103 3.32±0.27* 2.43±0.51* 与MAP2K4检测比较, *P < 0.05。 
下载: 导出CSV
表 2 MAP2K4与波形蛋白表达及临床病理因素的相关性(n=103)
项目 n MAP2K4检测 波形蛋白检测 阳性 阴性 χ2 P 阳性 阴性 χ2 P 年龄 < 50岁 36 13(36.11) 23(63.89) 0.072 0.788 21(58.33) 15(41.67) 1.098 0.295 ≥50岁 67 26(38.81) 41(61.19) 46(68.66) 21(31.34) 绝经情况 是 49 17(34.69) 32(65.31) 0.399 0.528 29(59.18) 20(40.82) 1.414 0.234 否 54 22(40.74) 32(59.26) 38(70.37) 16(29.63) FIGO分期 Ⅰ 81 36(44.44) 45(55.56) 6.980 0.008 48(59.26) 33(40.74) 5.590 0.018 Ⅱ、Ⅲ 22 3(13.64) 19(86.36) 19(86.36) 3(13.64) 组织学分型 高分化 47 22(46.81) 25(53.19) 3.552 0.169 25(53.19) 22(46.81) 7.042 0.030 中分化 35 12(34.29) 23(65.71) 24(68.57) 11(31.43) 低分化 21 5(23.81) 16(76.19) 18(85.71) 3(14.29) 浸润深度 <1/2 87 35(40.23) 52(59.77) 1.332 0.248 53(60.92) 34(39.08) 4.200 0.040 ≥1/2 16 4(25.00) 12(75.00) 14(87.50) 2(12.50) 淋巴转移 是 12 1(8.33) 11(91.67) 5.035 0.025 11(91.67) 1(8.33) 4.233 0.040 否 91 38(41.76) 53(58.24) 56(61.54) 35(38.46)
下载: 导出CSV
-
[1] 刘春花, 江庆萍, 林丹, 等. 子宫内膜癌中MAP2K4和波形蛋白的表达及临床意义[J]. 南方医科大学学报, 2017, 37(2): 157-164. doi: 10.3969/j.issn.1673-4254.2017.02.03 [2] Wang C I, Wang C L, Wu Y C, et al. Quantitative proteomics reveals a novel role of karyopherin alpha 2 in cell migration through the regulation of vimentin-pErk protein complex levels in lung cancer[J]. J Proteome Res, 2015, 14(4): 1739-1751. doi: 10.1021/pr501097a
[3] 龚惠, 金玉华, 何萍, 等. 波形蛋白的表达与子宫内膜癌患者术后预后的关系[J]. 现代生物医学进展, 2017, 17(33): 6460-6464, 6490. https://www.cnki.com.cn/Article/CJFDTOTAL-SWCX201733013.htm [4] Kamphuis W, Kooijman L, Orre M, et al. GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive Glia in mouse model for Alzheimer's disease[J]. Glia, 2015, 63(6): 1036-1056. doi: 10.1002/glia.22800
[5] 张勇, 银铎. 波形蛋白在妇科恶性肿瘤中的研究进展[J]. 肿瘤研究与临床, 2018, 30(3): 207-211. doi: 10.3760/cma.j.issn.1006-9801.2018.03.017 [6] 常磊, 袁玉峰, 郭涛, 等. 波形蛋白、E-钙黏蛋白在原发性肝癌上皮间质转化过程中的表达及其意义[J]. 中华肝胆外科杂志, 2015, 21(1): 9-13. doi: 10.3760/cma.j.issn.1007-8118.2015.01.003 [7] 聂嘉, 张博, 顾斌, 等. p38丝裂原活化蛋白激酶在炎症微环境作用下对牙周膜干细胞成骨分化的影响[J]. 中国医学科学院学报, 2015, 37(1): 1-7. doi: 10.3881/j.issn.1000-503X.2015.01.001 [8] 王露, 罗科, 卿国忠, 等. 沐舒坦预处理对小鼠脓毒症所致肺损伤时p38丝裂原活化蛋白激酶通路的影响[J]. 中华急诊医学杂志, 2018, 27(3): 283-288. doi: 10.3760/cma.j.issn.1671-0282.2018.03.011 [9] 杨曦, 刘玉洁, 邢邯英, 等. 丝裂原活化蛋白激酶信号通路对软脂酸培养的肌细胞过氧化物酶体增殖物激活受体γ辅激活子表达的调控作用[J]. 中国糖尿病杂志, 2017, 25(11): 1016-1020. doi: 10.3969/j.issn.1006-6187.2017.11.012 [10] 王丽雪, 解玉怀, 杨维仁, 等. 植物多糖通过核因子κB及丝裂原活化蛋白激酶/核因子E2相关因子2信号通路发挥抗氧化作用的机制[J]. 动物营养学报, 2018, 30(10): 3857-3863. doi: 10.3969/j.issn.1006-267x.2018.10.009 [11] 金子, 王秀丽, 吴川, 等. p38丝裂原活化蛋白激酶通路对紫杉醇诱发细胞凋亡中γ-氨基丁酸B型受体表达变化的影响[J]. 中华实验外科杂志, 2015, 32(3): 546-549. doi: 10.3760/cma.j.issn.1001-9030.2015.03.037 [12] 刘蜀, 刘祎祎, 李荣. 丝裂原活化蛋白激酶激酶4和雌激素受体的表达在浸润性乳腺癌组织中的临床意义[J]. 南方医科大学学报, 2017, 37(4): 488-493. doi: 10.3969/j.issn.1673-4254.2017.04.11 [13] 吕嘉, 宋力雯, 丘瑾, 等. 雌激素受体孕激素受体和波形蛋白在子宫内膜癌的表达及临床意义[J]. 山西医药杂志, 2015, 44(24): 2843-2846. https://www.cnki.com.cn/Article/CJFDTOTAL-SXYY201524001.htm [14] Yao W J, Gu C J, Shao H R, et al. Tetrahydroxystilbene glucoside improves TNF-α-induced endothelial dysfunction: involvement of TGFβ/Smad pathway and inhibition of vimentin expression[J]. Am J Chin Med, 2015, 43(1): 183-198. doi: 10.1142/S0192415X15500123
[15] 张競. 子宫内膜癌组织中MAP2K4、pRb2/p130的表达变化及其意义[J]. 山东医药, 2017, 57(23): 72-74. doi: 10.3969/j.issn.1002-266X.2017.23.022 [16] Lee J, Hahm E R, Marcus A I, et al. Withaferin A inhibits experimental epithelial-mesenchymal transition in MCF-10A cells and suppresses vimentin protein level in vivo in breast tumors[J]. Mol Carcinog, 2015, 54(6): 417-429. doi: 10.1002/mc.22110
计量
- 文章访问数: 161
- HTML全文浏览量: 72
- PDF下载量: 3
苏公网安备 32100302010246号
