表没食子儿茶素没食子酸酯对病毒性心肌炎小鼠的心肌保护作用

曾闵; 陈智; 易世江; 王野峰; 王勋

doi:10.7619/jcmp.201912025

表没食子儿茶素没食子酸酯对病毒性心肌炎小鼠的心肌保护作用

1.
湖南省儿童医院心血管内科, 湖南长沙, 410007
2.
桂林医学院, 广西桂林, 541001

详细信息

中图分类号: R542.2
计量
- 文章访问数: 226
- HTML全文浏览量: 135
- PDF下载量: 7
出版历程
- 收稿日期: 2019-04-16
- 录用日期: 2019-05-24
- 网络出版日期: 2021-02-22
- 发布日期: 2019-06-27

Role of epigallocatechin gallate in protecting myocardium of rats with viral myocarditis

1.
Department of Cardiovascular Medicine, Hunan Children′s Hospital, Changsha, Hunan, 410007
2.
Guilin Medical University, Guilin, Guangxi, 541001

摘要

摘要:
目的探讨表没食子儿茶素没食子酸酯(EGCG)对病毒性心肌炎小鼠的心肌保护作用。
方法将45只雄性BALA/C小鼠随机分为正常组、对照组及EGCG组, 每组15只。对照组、EGCG组小鼠腹腔注射0.1 mL CVB3病毒液。接种成功后, EGCG组予以EGCG 100 mg/(kg·d)灌胃处理，对照组予以等量的生理盐水灌胃。正常组小鼠腹腔注射等量的生理盐水。饲养1周后，摘除眼球取血检测肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白T(cTnT)的活性，然后处死动物取出心脏, HE染色观察心肌细胞形态学改变。
结果 EGCG组小鼠的生存率显著高于对照组; 对照组血清CK-MB、cTnT的活性及心肌病理评分显著高于EGCG组。
结论 EGCG对病毒性心肌炎具有一定的保护作用。
- 表没食子儿茶素没食子酸酯 /
- 病毒性心肌炎 /
- 小鼠 /
- 肌酸激酶同工酶 /
- 心肌肌钙蛋白T
Abstract:
Objective To investigate the role of epigallocatechin gallate (EGCG) in protecting myocardium of rats with viral myocarditis.
Methods A total of 45 male BALA/C rats were randomly divided into normal group, control group and EGCG group, with 15 rats in each group. Rats in the control group and EGCG group were intraperitoneally injected with 0.1 mL CVB3 viral fluid. After successful inoculation, the EGCG group was treated with 100 mg/kg EGCG per day, while the control group was treated with the same amount of saline. Normal rats were injected with the same amount of saline. After feeding for one week, the eyeball blood was removed to detect the activity of creatine kinase isoenzyme (CK-MB) and cardiac troponin T (cTnT). Then the heart was taken out and the morphological changes of cardiac myocytes were observed by HE staining.
Results The survival rate of rats in the EGCG group was obviously higher than that in the control group, and the serum CK-MB, cTnT activity and myocardial pathological score in the control group were obviously higher than those in the EGCG group.
Conclusion EGCG has a certain protective effect on viral myocarditis.
- epigallocatechin gallate /
- viral myocarditis /
- rats /
- creatine kinase isoenzyme /
- cardiac troponin T

HTML全文

表 1 3组小鼠心肌病理评分比较(x±s) 分

组别	n	心肌坏死	炎性细胞浸润
正常组	15	0	0
对照组	15	1.67±0.12^*	2.79±0.15^*
EGCG组	15	0.59±0.08^*#	1.53±0.17^*#
与正常组比较, *P < 0.05; 与对照组比较, #P < 0.05。

下载: 导出CSV

表 2 小鼠血清中CK-MB、cTnT水平比较(x±s)

组别	n	CK-MB/(U/L)	cTnT/(pg/L)
正常组	15	83.00±10.80	0
对照组	15	182.00±11.90^**	2.76±0.10^**
EGCG组	15	153.00±12.80^**#	1.51±0.16^**#
cTnT: 心肌肌钙蛋白T; CK-MB: 肌酸激酶同工酶。与正常组比较, **P < 0.01; 与对照组比较, #P < 0.05。

下载: 导出CSV

参考文献(15)

[1]	Sang Y M, Gu X H, Pan L L, et al. Melatonin ameliorates coxsackievirus B3-induced myocarditis by regulating apoptosis and autophagy[J]. Front Pharmacol, 2018, 9: 1384-1389. doi: 10.3389/fphar.2018.01384
[2]	Hendry R G, Bilawchuk L M, Marchant D J. Targeting matrix metalloproteinase activity and expression for the treatment of viral myocarditis[J]. J of Cardiovasc Trans Res, 2014, 7(2): 212-225. doi: 10.1007/s12265-013-9528-2
[3]	王振先, 郝芳之, 徐成伟, 等. 复合蛋白锌对病毒致小鼠心肌损伤的保护作用[J]. 山东大学学报: 医学版, 2004, 42(1): 106-109. doi: 10.3969/j.issn.1671-7554.2004.01.032
[4]	Khan N, Mukhtar H. Tea polyphenols in promotion of human health[J]. Nutrients, 2018, 11(1): E39-E45. doi: 10.3390/nu11010039
[5]	Cen Z, Guo Y, Kong Q, et al. IL-10-producing B cells involved in the pathogenesis of Coxsackie virus B3-induced acute viral myocarditis[J]. International Journal of Clinical & Experimental Pathology, 2015, 8(1): 830-835. http://europepmc.org/articles/PMC4348857
[6]	Chacko S M, Thambi P T, Kuttan R, et al. Beneficial effects of green tea: a literature review[J]. Chin Med, 2010, 5: 13-19. doi: 10.1186/1749-8546-5-13
[7]	Yang C S, Wang H. Cancer preventive activities of tea catechins[J]. Molecules, 2016, 21(12): E1679-E1685. doi: 10.3390/molecules21121679
[8]	Xu J, Xu Z, Zheng W M. A review of the antiviral role of green tea catechins[J]. Molecules, 2017, 22(8): E1337-E1341. doi: 10.3390/molecules22081337
[9]	Ide K, Kawasaki Y, Kawakami K, et al. Anti-influenza virus effects of catechins: a molecular and clinical review[J]. Curr Med Chem, 2016, 23(42): 4773-4783. doi: 10.2174/0929867324666161123091010
[10]	Xu J, Wang J, Deng F, et al. Green tea extract and its major component epigallocatechin gallate inhibits hepatitis B virus in vitro[J]. Antiviral Res, 2008, 78(3): 242-249.
[11]	Calland N, Albecka A, Belouzard S, et al. Epigallocatechin-3-gallate is a new inhibitor of hepatitis C virus entry[J]. Hepatology, 2012, 55(3): 720-729. doi: 10.1002/hep.24803
[12]	Lyu S Y, Rhim J Y, Park W B. Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in vitro[J]. Arch Pharm Res, 2005, 28(11): 1293-1301. doi: 10.1007/BF02978215
[13]	钱净, 李雪, 杨丽琼, 等. 常见心肌损伤标志物在急性心肌梗死诊断中的应用价值[J]. 检验医学与临床, 2019, 16(1): 31-34. doi: 10.3969/j.issn.1672-9455.2019.01.009
[14]	胡孝彬, 向小节, 郭晓聪, 等. 高敏肌钙蛋白T对儿童病毒性心肌炎诊断价值探讨[J]. 检验医学, 2014, 29(4): 307-311. doi: 10.3969/j.issn.1673-8640.2014.04.002
[15]	He X R, Gao B, Zhou L, et al. Green tea polyphenol epigallocatechin-3-gallate-alleviated coxsackievirus B3-induced myocarditis through inhibiting viral replication but not through inhibiting inflammatory responses[J]. J Cardiovasc Pharmacol, 2017, 69(1): 41-47. http://www.ncbi.nlm.nih.gov/pubmed/27753702

施引文献

资源附件(0)

表(2)

计量

文章访问数: 226
HTML全文浏览量: 135
PDF下载量: 7
被引次数: 0

表没食子儿茶素没食子酸酯对病毒性心肌炎小鼠的心肌保护作用

计量

出版历程

Role of epigallocatechin gallate in protecting myocardium of rats with viral myocarditis

计量

出版历程

目录