Experimental study on apoptosis of gastric cancer cell line SGC-7901 induced by coix seed oil
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摘要:目的 探讨薏苡仁活性成分诱导胃癌细胞(SGC-7901)凋亡的可能机制。方法 将不同浓度的薏苡仁油(2、4、8 mg/mL)作用于SGC-7901细胞, 采用MTT法检测药物对细胞增殖的影响，流式细胞术检测药物诱导细胞的凋亡，划痕实验检测薏苡仁油抑制细胞的迁移, Transwell小室检测药物抑制细胞的侵袭, Western blot检测相关蛋白PRMT5、PI3K和AKT的表达。结果 MTT结果显示, 2、4 mg/mL的薏苡仁油能显著抑制SGC-7901细胞的增殖, 2 mg/mL薏苡仁油抑制率达(30.02±1.56)%, 与空白对照组相比差异极显著(P < 0.01)。流式细胞实验结果表明, 2、4 mg/mL的薏苡仁油细胞凋亡率分别为(16.25±2.54)%、(12.60±1.12)%, 与空白对照组(2.00±1.22)%相比差异极显著(P < 0.01)。细胞划痕实验结果表明, 2、4 mg/mL的薏苡仁油处理过的SGC-7901细胞迁移缓慢，与空白对照组间距对比差异极显著(P < 0.01)。侵袭实验结果表明, 2、4 mg/mL的薏苡仁油可显著抑制细胞的侵袭，细胞侵袭数分别是(134.00±2.86)、(167.00±0.99)个，与对照组的(268.00±2.05)个相比，差异极显著(P < 0.01), 8 mg/mL组的迁移数为(203.00±2.97)个，与对照组对比差异显著(P < 0.05)。Western blot结果显示，不同浓度的薏苡仁油能够显著下调SGC-7901细胞中PRMT5、PI3K和AKT的表达。结论 薏苡仁油能够显著抑制胃癌SGC-7901细胞的增殖、迁移与侵袭，其机制可能是通过下调PRMT5-PI3K/AKT信号通路，阻断下游多种抗凋亡分子的活化，诱导细胞凋亡、抑制胃癌SGC-7901细胞的侵袭与转移。
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关键词:
- 薏苡仁油 /
- 胃癌 /
- 蛋白质精氨酸甲基转移酶5 /
- 迁移
Abstract:Objective To investigate the possible mechanism of coix seed in inducing apoptosis of gastric cancer cells (SGC-7901).Methods Different concentrations of coix seed oil (2, 4, 8 mg/mL) were applied to SGC-7901 cells. MTT assay was used to detect the effect of drugs on cell proliferation, and flow cytometry for drug-induced cell apoptosis, scratch test for cell migration inhibited by coix seed oil, Transwell chamber for drug-inhibited cell invasion, and Western blot for expression of related proteins PRMT5, PI3K and AKT.Results The results of MTT showed that 2, 4 mg/mL of coix seed oil could significantly inhibit the proliferation of SGC-7901 cells, and the inhibition rate of 2 mg/mL was (30.02±1.56)%, which showed significant difference compared to the control group (P < 0.01). The results of flow cytometry showed that the apoptosis rates of coix seed oil cells at concentrations of 2 and 4 mg/mL were (16.25±2.54)%, (12.60±1.12)%, respectively, and which showed a significant difference compared with (2.0±1.22)% in the control group (P < 0.01). The results of cell scratch test showed that the migration of SGC-7901 cells treated with 2, 4 mg/mL of coix seed oil had significant difference when compared to control group (P < 0.01). The results of invasion experiments showed that 2, 4 mg/mL of coix seed oil could significantly inhibit cell invasion, and the number of cell invasion was(134.00±2.86), (167.00±0.99), respectively, which showed significant difference compared to(268.00±2.05)in the control group (P < 0.01). The migration number in 8 mg/mL coix seed oil group was (167±0.99), a significant difference was observed when compared to the control group(P < 0.05). Western blot analysis showed that different concentrations of coix seed oil could significantly down-regulate the expression of PRMT5, PI3K and AKT in SGC-7901 cells.Conclusion Coix seed oil can significantly inhibit the proliferation, migration and invasion of gastric cancer SGC-7901 cells, its possible mechanism is to down-regulate the signal pathway of PRMT5-PI3K/AKT to inhibit the activation of various anti-apoptotic molecules, induce apoptosis and suppress invasion and metastasis of gastric cancer SGC-7901 cells by down-regulating the PRMT5-PI3K/AKT signaling pathway.-
Keywords:
- coix seed oil /
- gastric cancer /
- protein arginine methyltransferase 5 /
- migration
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图 3 薏苡仁油抑制SGC-7901细胞的迁移(100倍)
A: 0 mg/mL; B: 2 mg/mL; C: 4 mg/mL; D: 8 mg/mL。与0 mg/mL相比, *P < 0.05, **P < 0.01。
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图 4 薏苡仁油抑制SGC-7901细胞的侵袭(100倍)
A: 0 mg/mL; B: 2 mg/mL; C: 4 mg/mL; D: 8 mg/mL。与0 mg/mL相比, *P < 0.05, **P < 0.01。
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