The clinical significance of detecting MMP-2mRNA and MMP-14mRNA with peritoneal lavage fluid in patients with gastric cancer
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摘要: 目的：探讨胃癌患者术中腹腔冲洗液的 MMP-2mRNA、MMP-14mRNA 在胃癌腹腔转移中的临床意义。方法应用 RT-PCR 法检查98例原发性胃癌腹腔冲洗液中 MMP-2mRNA、MMP-14mRNA 的表达，30例良性病变病人腹腔冲洗液作为阴性对照。结果胃癌患者腹腔冲洗液中MMP-2mRNA、MMP-14mRNA阳性表达率分别为42/98（42．9％）、40/98（40．8％），分别高于对照组的2/30（6．67％）、3．33％（1/30）（P ＜0．01）。MMP-2mRNA、MMP-14mRNA 的表达阳性率随着肿瘤浸润深度的加深而显著增加，与腹腔内是否转移及胃癌的大体类型有关（P ＜0．01），两者呈正相关。结论采用 RT-PCR技术检测胃癌患者腹腔冲洗液中的 MMP-2mRNA 及 MMP-14mRNA 表达，可以用于预测胃癌患者腹腔转移。同时也说明MMP-2及 MMP-14参与了胃癌的腹腔转移的过程。
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关键词:
- 胃癌 /
- 基质金属蛋白酶-2 /
- 基质金属蛋白酶-14 /
- 腹腔冲洗液 /
- 腹腔转移
Abstract: Objective To investigate the clinical significance of detecting MMP-2mRNA and MMP-14mRNA with peritoneal lavage fluid in patients with gastric cancer.Methods The ex-pression of 98 patients of MMP-2mRNA and MMP-14 mRNA in peritoneal lavage fluid were de-teced by RT-PCR in patients with gastric cancer.30 patients with benign peritoneal lavage fluid were in the negative control group.Results The positive expression rates of MMP-2mRNA and MMP-14mRNA in the peritoneal lavage fluid in patients with gastric cancer were 42 /98(42.9%) and 40 /98(40.8%).but they were 2 /30(6.67%)、3.33% (1 /30)respectively in normal control (P <0.01).The positive expression rate of MMP-2mRNA and MMP-14mRNA were related to the depth of invasion,peritoneal metastasis,macroscopic type (P <0.01).Conclusion RT-PCR de-tection on the expression of MMP-2mRNA and MMP-14mRNA in peritoneal lavage fluid in patients with gastric cancer can be used to predict the peritoneal metastases.MMP-2 and MMP-14 may be involved in the peritoneal metastasis process. -
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