重组人血管内皮抑素联合顺铂体外干预人胃癌细胞生长的研究

邓建良, 谈华阳, 王维民, 周炎, 许春妮, 张国强

邓建良, 谈华阳, 王维民, 周炎, 许春妮, 张国强. 重组人血管内皮抑素联合顺铂体外干预人胃癌细胞生长的研究[J]. 实用临床医药杂志, 2014, (1): 42-45. DOI: 10.7619/jcmp.201401013
引用本文: 邓建良, 谈华阳, 王维民, 周炎, 许春妮, 张国强. 重组人血管内皮抑素联合顺铂体外干预人胃癌细胞生长的研究[J]. 实用临床医药杂志, 2014, (1): 42-45. DOI: 10.7619/jcmp.201401013
DENG Jianliang, TAN Huayang, WANG Weimin, ZHOU Yan, XU Chunni, ZHANG Guoqiang. Research of recombinant human endostatin combined with cisplatin on extracorporeal intervention of growth of human gastric carcinoma cell[J]. Journal of Clinical Medicine in Practice, 2014, (1): 42-45. DOI: 10.7619/jcmp.201401013
Citation: DENG Jianliang, TAN Huayang, WANG Weimin, ZHOU Yan, XU Chunni, ZHANG Guoqiang. Research of recombinant human endostatin combined with cisplatin on extracorporeal intervention of growth of human gastric carcinoma cell[J]. Journal of Clinical Medicine in Practice, 2014, (1): 42-45. DOI: 10.7619/jcmp.201401013

重组人血管内皮抑素联合顺铂体外干预人胃癌细胞生长的研究

基金项目: 中国高校医学期刊临床专项资金
详细信息
  • 中图分类号: R735.2

Research of recombinant human endostatin combined with cisplatin on extracorporeal intervention of growth of human gastric carcinoma cell

  • 摘要: 目的 研究重组人血管内皮抑制素(恩度)联合顺铂对人胃癌细胞株BGC823和SGC7901增殖及凋亡的影响,并探讨其抑制增殖及诱导凋亡的机制.方法 采用四甲基偶氮唑盐(MTT)法检测不同浓度顺铂单药(单药组)及其与恩度联合应用(联合组)对BGC823和SGC7901细胞的增殖抑制率;采用流式细胞术分析凋亡细胞比例;采用Western blot法分析增殖相关蛋白Ki-67及凋亡相关蛋白Bcl-2表达的变化.结果 联合组对BGC823和SGC7901细胞的抑制率明显高于单药组;流式细胞术结果表明,正常对照组、单药组和联合组细胞的早期凋亡率依次增加;与单药组比较,联合组Ki-67、Bcl-2基因(蛋白)表达率显著降低.结论 与顺铂单独应用相比,恩度联合顺铂对BGC823和SGC7901细胞增殖的抑制作用和诱导凋亡能力均明显增强.
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出版历程
  • 发布日期:  2014-07-09

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