结直肠癌术后化疗药物5-氟尿嘧啶、奥沙利铂和亚叶酸钙的测定

陈锐; 阿依夏木; 马玉花; 丁远大; 封顺

doi:10.7619/jcmp.201315008

结直肠癌术后化疗药物5-氟尿嘧啶、奥沙利铂和亚叶酸钙的测定

1.
新疆维吾尔自治区人民医院,新疆乌鲁木齐,830001;
2.
新疆大学化学化工学院,新疆乌鲁木齐,830046

基金项目: 国家自然科学基金资助项目，新疆维吾尔自治区教育厅重点项目

详细信息

中图分类号: R735.3
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- 发布日期: 2013-10-15

Separation of 5-fluorouracil, leucovorin calcium and oxaliplatin based on HPLC method

摘要

摘要: 目的结直肠癌患者尿液中5-氟尿嘧啶、奥沙利铂和亚叶酸钙3种常用药物含量的测定.方法对4名结直肠癌患者尿样中5-氟尿嘧啶、奥沙利铂和亚叶酸钙3种药物残留量采用高效液相色谱法进行分离和分析,流速1 mL/min,流动相,pH=3磷酸水溶液/甲醇(80/20 v/v),检测波长270 nm,色谱柱Inertsil ODS(150 mm× 4.6 mm,5μm).结果 5-氟尿嘧啶、奥沙利铂和亚叶酸钙的检出限分别为42.0、29.4、33.3 μg/L,线性范围1.0～1 000.0 mg/L,相对标准偏差(RSD)分别为0.34％、0.98％、1.00％,回收率93.40％～～110.70％.结论该方法样品处理简便,色谱分离完全,结果准确可靠,为结直肠癌患者尿液中的药物代谢组学研究提供了基础.
- 结直肠癌 /
- 尿液 /
- 高效液相色谱法 /
- 5-氟尿嘧啶 /
- 奥沙利铂 /
- 亚叶酸钙
- colorectal cancer /
- urine /
- HPLC /
- 5-fluorouracil /
- oxaliplatin /
- leucovorin calcium

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参考文献(20)

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文章访问数: 276
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结直肠癌术后化疗药物5-氟尿嘧啶、奥沙利铂和亚叶酸钙的测定

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Separation of 5-fluorouracil, leucovorin calcium and oxaliplatin based on HPLC method

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