与白血病骨髓间充质干细胞共培养后K562细胞增殖与凋亡的变化

魏朝辉; 贾颖; 陈乃耀; 徐学新; 蒙艳凤; 张文军

doi:10.7619/jcmp.201311011

与白血病骨髓间充质干细胞共培养后K562细胞增殖与凋亡的变化

1.
河北省唐山市乐亭县医院内四科,河北唐山,063600;
2.
河北省唐山市乐亭县医院内二科,河北唐山,063600;
3.
河北联合大学附属医院血液科,河北唐山,063000

基金项目: 中国高校医学期刊临床专项资金

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中图分类号: R331.1
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- 发布日期: 2013-10-14

Changes of proliferation and apoptosis of K562 cells after cultivation with leukemia bone marrow mesenchymal stem cells

摘要

摘要: 目的比较K562细胞与人白血病骨髓间充质干细胞(LMSC)共培养前后增殖和凋亡的变化.方法采用细胞计数试剂盒-8(CCK-8)分析法检测SCG和CCG组K562细胞光密度(0D)值,比较不同培养条件下K562细胞的增殖情况.采用流式细胞仪检测与LMSC共培养后K562细胞周期变化,Annexin V/聚酰亚胺(PI)荧光标记法检测血清饥饿后与LMSC共培养后K562细胞凋亡的变化.结果与LMSC共培养后,K562细胞的增殖受到明显抑制,CCG组OD值明显低于SCG组.流式细胞仪检测细胞周期发现,与LMSC共培养后,G0～G1期细胞比例明显增高,S期细胞比例明显下降,且G2 ～M期细胞比例也呈上升趋势；CCG +0％FBS组K562细胞凋亡较SCG+ 10％ FBS组明显增多,较SCG+ 0％ FBS明显降低,LMSC有抵抗白血病细胞凋亡的作用.结论 LMSC通过阻滞G0～ G1期K562细胞周期而发挥抑制增殖的作用,并能抑制血清饥饿诱导的K562细胞凋亡.
- K562细胞 /
- 人白血病骨髓间充质干细胞 /
- 细胞增殖 /
- 细胞周期 /
- 细胞凋亡
- K562 cells /
- human leukemia bone marrow mesenchymal stem cells /
- cell proliferation /
- cell cycle /
- apoptosis

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参考文献(12)

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文章访问数: 142
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与白血病骨髓间充质干细胞共培养后K562细胞增殖与凋亡的变化

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出版历程

Changes of proliferation and apoptosis of K562 cells after cultivation with leukemia bone marrow mesenchymal stem cells

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出版历程

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