促红细胞生成素对大鼠体外循环后肾脏保护作用的研究

胡健; 殷仕宝; 叶阿奇; 钱云; 李德闽

doi:10.7619/jcmp.201309003

促红细胞生成素对大鼠体外循环后肾脏保护作用的研究

1.
江苏省仪征市人民医院,江苏仪征,211400;
2.
南京军区南京总医院,江苏南京,210002

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中图分类号: R692
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- 发布日期: 2013-10-17

Protective effect of erythropoietin on renal injury following cardiopulmonary bypass in rats

摘要

摘要: 目的研究促红细胞生成素(EPO)对于大鼠体外循环(CPB)术后肾脏损伤的保护作用并探讨其相关机制.方法将30只雄性SD大鼠随机均分为Sham组、CPB组及EPO组,每组10只.Sham组建立CPB模型管道,不进行CPB;其他2组建立CPB,以最大流量≥100 mL/(kg·min)转流维持1h,其中EPO组于转流前在预充液中加入EPO 3000U/kg.分别于肝素化后转流前(T0)、转流结束后(T1)和转流结束后0.5 (T2)、1(T3)、2(T4)、24 h(T5)检测血清肌酐(SCr)、尿素氮(BUN)水平；T5时取肾脏组织,检测组织肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)等炎症因子水平；同时测定肾脏组织丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)等氧化应激指标水平,并行HE染色观察组织形态学改变.结果 T0时3组SCr、BUN水平无显著差异；与CPB组相比,EPO组T1至T5各时间点SCr、BUN水平明显下降(P＜0.05),肾组织中TNF-a、IL-1β、IL-6等炎症因子水平均明显低于CPB组(P ＜0.05)；CPB组MDA高于Sham组,CAT、SOD活性则较Sham组低；EPO组CAT、SOD活性高于Sham组；组织病理学检查显示,EPO组的肾小管上皮细胞肿胀、胞浆内空泡形成、间质出血等病理变化较CPB组明显减轻(P＜0.05).结论 EPO可能通过抑制炎症因子的表达,降低氧化应激损伤,达到减轻CPB后肾脏损伤的目的.
- 促红细胞生成素 /
- 体外循环 /
- 肾脏损伤 /
- 炎症反应 /
- 氧化应激
- erythropoietin /
- cardiopulmonary bypass /
- renal injury /
- inflammatory response /
- oxidative stress

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参考文献(12)

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文章访问数: 262
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促红细胞生成素对大鼠体外循环后肾脏保护作用的研究

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出版历程

Protective effect of erythropoietin on renal injury following cardiopulmonary bypass in rats

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