Effects of sivelestat sodium on canine myocardial ischemia/reperfusion injury during cardiopulmonary bypass
-
摘要: 目的 探讨中性粒细胞弹性蛋白酶(NE)抑制剂西维来司钠对犬体外循环(CPB)中促炎性细胞因子IL-8和IL-1β引起的心肌缺血再灌注损伤的保护效果.方法 将12只犬随机分为C组(n=6)和S组(n=6),建立CPB心肌缺血再灌注损伤模型.S组于CPB前静脉注射西维来司钠40 mg/kg,C组静脉注射等量生理盐水.分别于CPB前及转机后不同时点采集静脉血,检测炎性因子的浓度;于阻断前后分别取心肌标本进行丙二醛(MDA)和髓过氧化物酶(MPO)活性检测;于阻断前,开放30、60 min测定血流动力学指标.结果 阻断60 min后,2组NE、IL-8和IL-1β浓度均显著升高(P<0.01);阻断60 min及开放主动脉后,S组中性粒细胞数、NE、IL-8和IL-1β浓度显著低于C组(P<0.01);与C组比较,S组阻断60 min及开放主动脉60min时心肌组织匀浆MDA和MPO均显著降低(P<0.01),S组在开放主动脉30、60min时血流动力学指标恢复迅速(P<0.01).结论 CPB中应用西维来司钠可明显减轻促炎性细胞因子和中性粒细胞介导的心肌缺血再灌注损伤.
-
-
Fujii M, Miyagi Y, Bessho R. Effect of a neutrophil elastase inhibitor on acute lung injury after cardiopulmonary bypass [J]. Interact Cardiovasx Thorac Surg, 2010(6):859. Ozkara C, Guler N, Kutay V. Leucocyte-depleted blood cardioplegia [J]. Journal of International Medical Research, 2007(2):188. Toyama S, Hatori F, Shimizu A. A neutrophil elastase inhibitor, sivelestat, improved respiratory and cardiac function in pediatric cardiovascular surgery with cardiopulmonary bypass [J]. Journal of Anesthesia, 2008(4):341. Ohata T, Mitsuno M, Yamamura M. Minimal cardiopulmonary bypass attenuates neutrophil activation and cytokine release in coronary artery bypass grafting [J]. Journal of Artificial Organs, 2007(2):92. Bical O M, Fromes Y, Gaillard D. Comparison of the inflammatory response between miniaturized and standard CPB circuits in aortic valve surgery [J]. European Journal of Cardio-Thoracic Surgery, 2006(5):699. Kotani T, Kotake Y, Morisaki H. Activation of a neutrophil-derived inflammatory response in the airways during cardiopulmonary bypass [J]. Anesthesia and Analgesia, 2006(6):1394. Wakayama F, Fukuda I, Suzuki Y. Neutrophil elastase inhibitor, sivelestat, attenuates acute lung injury after cardiopulmonary bypass in the rabbit endotoxemia model [J]. Annals of Thoracic Surgery, 2007(1):153. Kawabata K, Hagio T, Matsuoka S. Pharmacological profile of a specific neutrophil elastase inhibitor, Sivelestat sodium hydrate [J]. Nippon Yakuringaku Zasshi, 2003(2):151.
计量
- 文章访问数: 151
- HTML全文浏览量: 22
- PDF下载量: 9