Expression of NF-κB after hypertensive intracerebral hemorrhage in rats
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摘要: 目的 观察高血压性大鼠脑出血后血肿周围组织核因子-κB(NF-κB)的表达,分析其与出血时间的关系.方法 取成年SD大鼠80只,体质量250 ～ 300 g,大鼠高血压性脑出血模型采用双侧肾动脉前支部分热凝,立体定位仪下自体血脑内注入法建立.随机分为对照组和脑出血组,采用电泳迁移率改变分析方法(EMSA)检测核内NF-κB的活性,用免疫组化方法观察NF-κB在细胞内的表达情况.结果 大鼠高血压性脑出血后1h后NF-κB即开始表达,24h达到高峰,持续至72 h开始下降.结论 高血压性脑出血后早期即有NF-κB的表达,并参与了脑出血后周围脑组织的继发性损伤.
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