纳米SiO2对大鼠肺氧化损伤的研究

宋利海; 曹福源; 李健

doi:10.3969/j.issn.1672-2353.2011.24.002

纳米SiO2对大鼠肺氧化损伤的研究

1.
河北省乐亭县医院CT室,河北唐山,063600;
2.
河北联合大学毒理教研室,河北唐山,063000;
3.
河北联合大学附属开滦医院CT室,河北唐山,063000

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中图分类号: TB383 Q563
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- 发布日期: 2012-04-19

Nano SiO2 oxidative damage to the lungs in rats

摘要

摘要: 目的探讨纳米SiO2对大鼠肺氧化损伤作用机制.方法将24只体质量200 g左右的雄性大鼠随机分成3组(对照组、微米SiQ组和纳米SiO2组),每组8只.用一次性气管注入法,分别给予1mL的50 mg/mL石英粉尘(微米SiO2)混悬液、1mL的50 mg/mL纳米SiO2混悬液、1 mL生理盐水.30 d后进行肺泡灌洗,测定2种粉尘染尘后大鼠肺泡灌洗液(BALF)中的巨噬细胞凋亡率、总蛋白含量、总抗氧化能力(T - AOC)、丙二醛(MDA)含量、羟脯氨酸(HYP)含量；观察肺脏病理形态学改变；采用免疫组织化学法检测肺组织中转化生长因子β-1 (TGF- β1)和白介素-4(IL -4)蛋白的表达.结果微米SiO2组和纳米SiO2组巨噬细胞凋亡率高于对照组；纳米SiO2组凋亡率高于微米SiO2组,差异有统计学意义.微米SiO2组及纳米SiO2组较对照组MDA、HYP和总蛋白含量均增加,T-AOC下降.免疫组化结果显示:微米SiO2组TGF-β1及IL-4表达明显高于纳米SiO2组和对照组.结论纳米SiO2能够对肺组织造成一定的氧化损伤,但其氧化损伤并不是肺纤维化的主要因素.
- 纳米 /
- TGF-β1 /
- IL-4 /
- 肺纤维化 /
- 氧化损伤

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参考文献(8)

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文章访问数: 157
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纳米SiO2对大鼠肺氧化损伤的研究

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Nano SiO2 oxidative damage to the lungs in rats

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