A study of tumor suppressor gene PML mutation in cervical lesions
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摘要: 目的 探讨抑癌基因早幼粒细胞性白血病基因(PML)在宫颈病变组织中是否存在突变.方法 随机选取以下宫颈病变新鲜组织标本,包括宫颈上皮内瘤样病变I级(CIN Ⅰ)、CIN Ⅱ、CIN Ⅲ、宫颈浸润鳞癌各30例、以及因其他疾病切除子宫病理证实为慢性宫颈炎者30例.用变性高效液相色谱(DHPLC)进行PML基因全部外显子及内含子-外显子交接区突变的筛查,对其中10例宫颈浸润鳞癌组织进行PML基因全部外显子及内含子-外显子交接区测序.结果 全部组织均未发现PML基因突变.结论 PML在蛋白质水平的表达改变不是由其基因突变所引起.
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关键词:
- 抑癌基因 /
- 早幼粒细胞性白血病基因 /
- 宫颈病变 /
- 基因突变
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