整合蛋白β1 shRNA表达质粒的构建及其对肝癌细胞SMMC-7721增殖的影响

张振中; 张炜炜; 杨春霞; 朱珠; 傅奕

doi:10.3969/j.issn.1672-2353.2010.19.004

整合蛋白β1 shRNA表达质粒的构建及其对肝癌细胞SMMC-7721增殖的影响

1.
扬州大学医学院07级临床本科,江苏,扬州,225001;
2.
扬州大学医学院生物化学教研室,江苏,扬州,225001

基金项目: 国家自然科学基金资助项目，江苏省高校自然科学基金资助项目

详细信息

中图分类号: R735.7
计量
- 文章访问数: 180
- HTML全文浏览量: 23
- PDF下载量: 11
出版历程
- 发布日期: 2011-01-12

Construction of integrin β1 shRNA expression plasmid and its regulation on proliferation in cell line SMMC-7721

摘要

摘要: 目的应用RNAi技术,构建整合蛋白β1 shRNA表达质粒,诱导肝癌细胞中整合蛋白β1基因沉默,并观察其对肝癌细胞SMMC-7721增殖的影响.方法设计整合蛋白β1 shRNA序列,与载体pSilencer 2.0连接,构建整合蛋白β1 shRNA表达质粒.经测序鉴定后,转染肝癌细胞SMMC-7721, Western blot检测整合蛋白β1及p27的蛋白表达, MTT方法测定转染后细胞的增殖能力.结果成功构建了整合蛋白β1 shRNA表达质粒,转染肝癌细胞SMMC-7721后,整合蛋白β1的蛋白表达被明显抑制, p27的蛋白表达也随之减少.MTT实验结果发现转染细胞的增殖能力明显提高.结论应用RNAi技术,可特异性阻断整合蛋白β1的蛋白表达.整合蛋白β1可调节肝癌细胞的增殖,并可能与p27的表达相关.
- 整合蛋白β1 /
- shRNA /
- 肝癌细胞 /
- 细胞增殖

HTML全文

参考文献(10)

Giancotti FG. A structural view of integrin activation and signaling [J]. Developmental Cell, 2003(2):149.doi: 10.1016/S1534-5807(03)00034-0.

Harris ES, McIntyre TM, Prescott SM. The leukocyte integrins [J]. Journal of Biological Chemistry, 2000, (31):23409.doi: 10.1074/jbc.R000004200.

Yao M, Zhou XD, Zha XL. Expression of the integrin alpha5 subunit and its mediated cell adhesion in hepatocellular carcinoma [J]. Journal of Cancer Research and Clinical Oncology, 1997(8):435.doi: 10.1007/BF01372547.

Liang YL, Lei TW, Wu H. S-phase delay in human hepatocellular carcinoma cells induced by overexpression of integrin beta 1 [J]. World Journal of Gastroenterology, 2003(8):1689.

Miranti CK, Brugge JS. Sensing the environment:a historical perspective on integrin signal transduction [J]. Nature Cell Biology, 2002(4):E83.doi: 10.1038/ncb0402-e83.

Sherr CJ, Roberts JM. CDK inhibitors:positive and negative regulators of G1-phase progression [J]. Genes and development, 1999, (12):1501.doi: 10.1101/gad.13.12.1501.

Slingerland J, Pagano M. Regulation of the cdk inhibitor p27 and its deregulation in cancer [J]. Journal of Cellular Physiology, 2000(1):10.doi: 10.1002/(SICI)1097-4652(200004)183:1<10::AID-JCP2>3.0.CO;2-I.

Zhou GF, Ye F, Cao LH. Over expression of integrin alpha 5 beta 1 in human hepatocellular carcinoma cell line suppresses cell proliferation in vitro and tumorigenicity in nude mice [J]. Molecular and Cellular Biochemistry, 2000, (1-2):49.doi: 10.1023/A:1007034012642.

Hannon GJ. RNA interference [J]. Nature, 2002, (6894):244.doi: 10.1038/418244a.

Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells [J]. Science, 2002.550.doi: 10.1126/science.1068999.

施引文献(4)

期刊类型引用(3)

1.	付晓峰. 尿β2-MG、WBC水平检测对尿路感染的诊断价值分析. 辽宁医学杂志. 2022(04): 81-83 . 百度学术
2.	焦倩谦，刘斌. 尿LEU、NIT、HBP及BACT计数检测诊断尿路感染的临床分析. 中国校医. 2021(09): 688-689+692 . 百度学术
3.	任杰，范哲琦. 血清hs-CRP、PCT、尿β_2-微球蛋白检测诊断小儿尿路感染的临床分析. 中国校医. 2021(10): 796-798 . 百度学术

其他类型引用(1)

资源附件(0)

计量

文章访问数: 180
HTML全文浏览量: 23
PDF下载量: 11
被引次数: 4

整合蛋白β1 shRNA表达质粒的构建及其对肝癌细胞SMMC-7721增殖的影响

计量

出版历程

Construction of integrin β1 shRNA expression plasmid and its regulation on proliferation in cell line SMMC-7721

期刊类型引用(3)

其他类型引用(1)

计量

出版历程

目录