Growth inhibition effect of C225 on human nasopharyngeal carcinoma CNE cell line
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摘要: 目的 探讨EGFR单抗C225对人鼻咽癌细胞株CNE的生长抑制作用及可能机制.方法 以MTT比色法、倒置相差显微镜观察C225对CNE的生长抑制作用,以透射电镜、流式细胞术观察C225对CNE的凋亡诱导效应.结果 MTT法显示,C225对CNE具有明显的生长抑制作用,并随药物浓度的增加而逐渐增强,48 h IC20～30为10 μg/mL;以此浓度C225处理CNE,倒置相差显微镜观察发现,细胞变圆、脱壁,部分死亡、漂浮;透射电镜可见典型的凋亡超微结构改变;流式细胞术检测显示其24、48 h凋亡率分别为(14.30±0.78)%、(15.30±0.95)%,显著高于对照组的(2.90±0.95)%和(4.70±0.60)% (P＜0.05).结论 C225对CNE细胞有明显的生长抑制作用,这可能其与诱导CNE凋亡有关.
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