江苏省南京地区耐青霉素肺炎链球菌快速诊断方法初步探讨

丁晶晶; 张德平

doi:10.3969/j.issn.1672-2353.2010.05.013

江苏省南京地区耐青霉素肺炎链球菌快速诊断方法初步探讨

南京大学医学院附属鼓楼医院,呼吸科,江苏,南京,210008

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中图分类号: R563.1
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- 发布日期: 2010-05-30

摘要

摘要: 目的探讨本地区临床分离的肺炎链球菌青霉素耐药性的快速诊断方法.方法 2005～2007年,共搜集130株肺炎链球菌临床分离株和50株健康定殖株,采用optochin纸片和胆汁溶菌试验鉴定,测定最低抑菌浓度(MIC), 提取基因组DNA, PCR法测定其青霉素耐药性.结果本地区健康定殖株均对青霉素敏感,临床分离株青霉素敏感率、中介率、耐药率分别为25.4%、23.1%和51.5%, 儿童组耐药率65.6%而成人组耐药率11.8%.PCR法用于耐药性检测时,在青霉素敏感株中,最佳诊断标准为pbp2B、2X阳性, pbp1A阴性,敏感性、特异性分别为94.2%、96.8%; 而在青霉素不敏感株中,最佳诊断标准为pbp2X阴性,敏感性、特异性分别为97.8%、89.9%, 该法用于诊断中介株和耐药株敏感性较差,分别为26.1%、72.7%.用于检测的最低菌量为2～20 CFU.结论 PCR法检测时间明显短于传统药敏方法,且能够较好地区分出青霉素敏感株和耐药株,但不能理想地鉴定出中介株.
- 社区获得性肺炎(CAP) /
- 肺炎链球菌 /
- 青霉素 /
- PCR /
- 快速诊断

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参考文献(10)

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江苏省南京地区耐青霉素肺炎链球菌快速诊断方法初步探讨

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