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端粒酶hTERT促进肿瘤细胞侵袭与转移及其机制研究

陈陵, 余松涛, 梁光萍, 汤旭东, 房殿春, 杨仕明

陈陵, 余松涛, 梁光萍, 汤旭东, 房殿春, 杨仕明. 端粒酶hTERT促进肿瘤细胞侵袭与转移及其机制研究[J]. 实用临床医药杂志, 2010, (5): 13-20. DOI: 10.3969/j.issn.1672-2353.2010.05.003
引用本文: 陈陵, 余松涛, 梁光萍, 汤旭东, 房殿春, 杨仕明. 端粒酶hTERT促进肿瘤细胞侵袭与转移及其机制研究[J]. 实用临床医药杂志, 2010, (5): 13-20. DOI: 10.3969/j.issn.1672-2353.2010.05.003
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CHEN Ling, YU Song-tao, LIANG Guang-ping, TANG Xu-dong, FANG Dian-chun, YANG Shi-ming. hTERT promotes the invasion of telomerase-negative tumor cells in vitro and in vivo[J]. Journal of Clinical Medicine in Practice, 2010, (5): 13-20. DOI: 10.3969/j.issn.1672-2353.2010.05.003
Citation: CHEN Ling, YU Song-tao, LIANG Guang-ping, TANG Xu-dong, FANG Dian-chun, YANG Shi-ming. hTERT promotes the invasion of telomerase-negative tumor cells in vitro and in vivo[J]. Journal of Clinical Medicine in Practice, 2010, (5): 13-20. DOI: 10.3969/j.issn.1672-2353.2010.05.003
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端粒酶hTERT促进肿瘤细胞侵袭与转移及其机制研究

  • 1.

    第三军医大学西南医院全军消化病研究所,重庆,400038;

  • 2.

    第三军医大学西南医院全军烧伤研究所,重庆,400038

基金项目: 国家自然科学基金资助项目，重庆市杰出青年科学基金资助项目
详细信息

  • 中图分类号: R730.5

hTERT promotes the invasion of telomerase-negative tumor cells in vitro and in vivo

摘要

    摘要
  • 摘要: 目的 观察hTERT基因修饰对人骨肉瘤细胞系U-2 OS生物学行为的影响.方法 采用脂质体法将克隆有人全长cDNA hTERT的真核荧光质粒(pIRES2-EGFP-hTERT)转染端粒酶阴性的人骨肉瘤U-2 OS细胞,经G418筛选,免疫组化和Westorn Blot鉴定后,检测其端粒酶活性的改变、生长周期以及生物力学的变化.结果 成功转染人真核荧光表达载体的U-2 OS细胞能有效抵抗G418, 并可有效表达hTERT蛋白,细胞内端粒酶活性明显增强, G1期细胞比例下降, S期细胞比例升高,并且还明显增强了对细胞外基质的粘附力.进一步采用Transwell小孔迁移实验检测发现, hTERT/U2OS侵袭能力明显增强.结论 转染hTERT基因后, U-2 OS细胞内可同时通过端粒酶途径延长端粒.hTERT基因修饰可促进细胞周期进程,促使细胞从G1期→S期.通过替代途径延长端粒的肿瘤细胞在hTERT基因修饰后,增殖能力及侵袭能力明显增强.
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  • 发布日期:  2010-05-30

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