Correlation between Expression of NKg2D on lymphocytes and the Development of Colon Cancer
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摘要: 目的 探讨不同淋巴细胞亚群表面NKg2D受体的表达与结肠癌发病的关系.方法 首先收集10例结肠癌组织、外周血及相应肿瘤组织引流淋巴结和11例正常个体的外周血,分别用免疫组化检测肿瘤组织内NKg2D阳性淋巴细胞的数目,用流式细胞仪检测外周血及引流淋巴结内NKg2D阳性的总淋巴细胞数、CD8+T细胞数、γδT细胞数及NK细胞数.结果 结肠癌肿瘤组织内无NKg2D阳性的淋巴细胞,患者外周血NKg2D+NK细胞与正常个体相比明显减少(P=0.03).发生肿瘤转移的淋巴结内总淋巴细胞NKg2D的表达与同一个体外周血的相比,迅速降低(P=0.006),而且转移淋巴结内NKg2D+γδT细胞也明显减少(P=0.049).结论 结肠癌细胞可通过抑制NKg2D的表达而减弱机体的免疫功能.
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