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小鼠肝素酶H-2Kb限制性CTL表位的实验鉴定

汤旭东, 万瑛, 房殿春, 陈陵, 熊震, 余松涛, 师红磊, 郭军, 杨仕明

汤旭东, 万瑛, 房殿春, 陈陵, 熊震, 余松涛, 师红磊, 郭军, 杨仕明. 小鼠肝素酶H-2Kb限制性CTL表位的实验鉴定[J]. 实用临床医药杂志, 2008, (1): 5-11. DOI: 10.3969/j.issn.1672-2353.2008.01.002
引用本文: 汤旭东, 万瑛, 房殿春, 陈陵, 熊震, 余松涛, 师红磊, 郭军, 杨仕明. 小鼠肝素酶H-2Kb限制性CTL表位的实验鉴定[J]. 实用临床医药杂志, 2008, (1): 5-11. DOI: 10.3969/j.issn.1672-2353.2008.01.002
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TANG Xu-dong, WAN Yin, FANG Dian-chun, CHEN Ling, XIONG Zhen, YU Song-tao, SHI Hong-lei, GUO Jun, YANG Shi-ming. Identification of H-2Kb-restricted CTL epitopes derived from Heparanase antigen[J]. Journal of Clinical Medicine in Practice, 2008, (1): 5-11. DOI: 10.3969/j.issn.1672-2353.2008.01.002
Citation: TANG Xu-dong, WAN Yin, FANG Dian-chun, CHEN Ling, XIONG Zhen, YU Song-tao, SHI Hong-lei, GUO Jun, YANG Shi-ming. Identification of H-2Kb-restricted CTL epitopes derived from Heparanase antigen[J]. Journal of Clinical Medicine in Practice, 2008, (1): 5-11. DOI: 10.3969/j.issn.1672-2353.2008.01.002
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小鼠肝素酶H-2Kb限制性CTL表位的实验鉴定

  • 1.

    第三军医大学西南医院全军消化病研究所,重庆,400038;

  • 2.

    第三军医大学基础医学部全军免疫研究所,重庆,400038

基金项目: 国家自然科学基金
详细信息

  • 中图分类号: R730.5

Identification of H-2Kb-restricted CTL epitopes derived from Heparanase antigen

摘要

    摘要
  • 摘要: 目的 实验鉴定H-2Kb限制性小鼠肝素酶(mHpa)CTL表位.方法　5条mHpa表位分别负载C57BL/6小鼠骨髓来源树突状细胞,并进一步诱导产生表位特异性的效应细胞,采用标准4 hCr释放试验检测效应细胞对不同靶细胞的免疫杀伤效应;采用ELISPOT检测效应细胞分泌IFN-γ的能力.结果　5条mHpa CTL表位中,仅mHpa398-405和mHpa519-526可以诱导小鼠产生特异性CTL.对同来源的B16黑色素瘤细胞、Lewis肺癌细胞和EL-4淋巴瘤细胞具有明显的免疫杀伤效应,而对H-2Kb阴性的P815肥大细胞瘤细胞不具有免疫杀伤效应,进一步研究发现,上述多肽特异性CTL对自体淋巴细胞和DC细胞不具有杀伤效应,另一方面,mHpa398-405和mHpa519-526还可促进效应细胞分泌IFN-γ的能力.结论 mH-pa398-405、mHpa519-526为小鼠肝素酶来源且受仟12Kb限制性CTL表位,小鼠体内可以诱导产生表位特异性的CTL反应.
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  • 发布日期:  2008-07-13

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