EXPRESSION OF WT1 IN RATS WITH ADRIAMYCIN NEPHROPATHY
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摘要: 目的 观察WT1(Wilms' tumor 1)在大鼠阿霉素肾病模型中的表达,探讨其在蛋白尿发生发展过程中的作用.方法 30只SD大鼠随机分为模型组和对照组,建立阿霉素肾病模型,在第2、4、6、8周,应用光镜、电镜观察肾组织的病理改变, BCA法检测24 h尿蛋白排泄量,半定量Western Blot的方法检测大鼠肾皮质中WT1的表达.结果 ①随着模型组大鼠肾组织病理改变逐步加重及尿蛋白量逐渐增加,肾皮质WT1的表达量亦逐渐增加,第2、4、6、8周分别为对照组的1.5、2.7、3.1、4.3倍;② WT1的表达水平与尿蛋白量之间呈正相关性(P＜0.001).结论 阿霉素肾病模型大鼠肾皮质WT1的表达与大鼠蛋白尿的发生发展密切相关.
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