内皮抑素抑制SGC-7901生长的实验研究

孙振华; 陈平

doi:10.3969/j.issn.1672-2353.2006.03.015

内皮抑素抑制SGC-7901生长的实验研究

孙振华¹,
陈平²

1.
扬州大学医学院,江苏,扬州,225001;
2.
扬州大学临床医学院,江苏,扬州,225001

基金项目: 江苏省科技厅社会发展基金

详细信息

中图分类号: R735.2
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- 文章访问数: 270
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出版历程
- 发布日期: 2006-05-17

THE STUDY OF INHIBITING THE GROWTH OF SGC-7901 TUMOR CELL IMPLANTED IN NUDE MICE BY ENDOSTATIN

SUN Zhen-hua¹,
CHEN Pin²

摘要

摘要: 目的研究人内皮抑素抑制小鼠SGC-7901(胃腺癌)的生长和转移的作用.方法常规建立SGC-7901小鼠肿瘤动物模型.将荷瘤小鼠随机分成4组,对照组及治疗各组每组均为12只,共48只.对照组注射无菌生理盐水,治疗组分小、中、大剂量组,皮下注射内皮抑素,浓度分别为5、10、15 mg/kg, 注射次数均为1次/2 d, 共6周.第7周处死小鼠,测量原位肿瘤的直径、体积,计算抑瘤率;测定肿瘤细胞的增生指数及凋亡指数.结果内皮抑素治疗组(小、中、大剂量)能明显抑制肿瘤的生长,与对照组比较有显著性的差异(P《0.05);内皮抑素治疗组能降低肿瘤微血管密度,抑制肿瘤血管的形成,与对照组相比有显著性差异;治疗组肿瘤细胞凋亡指数明显上升,优于对照组(P《0.05).结论内皮抑素通过抗肿瘤血管生成,促进肿瘤细胞凋亡,对肿瘤的生长和转移均有较强的抑制作用.
- 胃癌 /
- 小鼠 /
- 内皮抑素 /
- SGC-7901 /
- 凋亡

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参考文献(15)

陈志琳, 徐立春, 孙振华. 抗肿瘤因子内皮抑素基因的克隆及表达 [J]. 扬州大学学报(自然科学版), 1999(4):136.doi: 10.3969/j.issn.1007-824X.1999.04.010.

Ponnazhagan S, Mahendra G, Kumar S. Adeno-associated virus 2-mediated antiangiogenic cancer gene therapy:Long-term efficacy of a vector encoding angiostatin and endostatin a single factor [J]. Cancer Research, 2004.1781.doi: 10.1158/0008-5472.CAN-03-1786.

Cao Y H, Veitonmaki N, Keough K. Elevated levels of urine angiostatin and plasminogen plamin in cancer patients [J]. International Journal of Molecular Medicine, 2000(5):547.

Hayes A J, Li L Y, Lippman M E. Science, medicene, and the future [J]. British Medical Journal, 1999.318.

Hohenester E, Sasaki T, Olsen R B. Crystal structure of the angiogenesis inhibitor endostatin at 1.5 A resolution [J]. EMBO Journal, 1998(6):1656.

Buscail L, Vemejoul F, Faure P. Regulation of cell proliferation by somatostatin [J]. Annales d'Endocrinologie, 2002(2):13.

Dhanabal M, Ramchandran R, Volk R. Endostatin:Yeast production, mutants, and antitumor effect in renal cell carcinoma [J]. Cancer Research, 1999(1):189.

Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease [J]. Nature Medicine, 1995(1):2731.

Kamphaus G D, Colorado P C, Panka D J. Canstatin, a novel martrix-derived inhibitor of angiogenesis and tumor growth [J]. Journal of Biological Chemistry, 2000(2):1209.doi: 10.1074/jbc.275.2.1209.

Boehm T, Folkman J, Browder T. Antiangiogenic therapy of experimatal cancer does not induce acquired drug resistance [J]. Nature, 1997, (6658):404.doi: 10.1038/37126.

Clark W H J. Model predicting survival in stage I melanoma based on tumor progression [J]. Journal of the National Cancer Institute, 1998.1893.doi: 10.1093/jnci/81.24.1893.

Hostikka S L, Tryggvason K. The complete primary structure of the plaha chain of human type Ⅳ collagen and comparison with the alpha 1(Ⅳ) chain [J]. Journal of Biological Chemistry, 1988.19488.

Sambrook J, Fritscj E F, Maniatis T. Molecular cloning:A laboratory manual [M]. New York:Cold Spring Harbor Laboratory Press, 1989.

Reilly M S, Holmlren L, Shing Y. Angiostatin:A novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma [J]. Cell, 1994.315.doi: 10.1016/0092-8674(94)90200-3.

Reilly M S, Boehm T, Shing Y. Endostatin:An endogenous inhibitor of angiogenesis and tumor growth [J]. Cell, 1997.277.doi: 10.1016/S0092-8674(00)81848-6.

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文章访问数: 270
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内皮抑素抑制SGC-7901生长的实验研究

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出版历程

THE STUDY OF INHIBITING THE GROWTH OF SGC-7901 TUMOR CELL IMPLANTED IN NUDE MICE BY ENDOSTATIN

计量

出版历程

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