EXPERIMENTAL STUDY OF INHIBITION OF OCTREOTIDE ON HUMAN GASTRIC CANCER CELLS GROWTH IN VITRO
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摘要: 目的探讨生长抑素类似物奥曲肽(OCT)对胃癌细胞株MKN45生长的体外调节作用.方法MTT法测定OCT对MKN45细胞增殖的调控;流式细胞术分析OCT对MKN45细胞周期分布的影响;RIA法测定表皮生长因子(EGF)、转化生长因子-α(TGF-α)含量的变化.结果OCT对MKN45细胞生长有抑制作用,以在1×10-6mol/L浓度时最明显;OCT可诱导MKN45细胞G0/G1阻滞,加入OCT后24 h最显著,同期G2/M期细胞比例亦减少;OCT能抑制胃癌细胞合成EGF、TGF-α的能力.结论OCT对胃癌生长具有负性调控作用,为胃癌患者应用OCT治疗提供了依据.
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