Biological effects of recombinant human interferon α on the proliferation of human scar fibroblasts
-
摘要: 目的 观察重组人α-干扰素(rHu IFN-α)对培养的人瘢痕成纤维细胞增殖的影响,探讨rHu IFN-α对瘢痕成纤维细胞生物学作用.方法 将rHu IFN-α加入瘢痕成纤维细胞培养基内,采用MTT法测定细胞增殖率,用3H-TdR掺入实验检测细胞DNA合成率,应用流式细胞仪分析细胞增殖周期.结果 当rHu IFN-α浓度>4000U/ml时,实验组OD值明显低于对照组(P<0.01).rHu IFN-α组3H-TdR掺入率明显下降,与DMEM组比差异显著(P<0.01).rHu IFN-α组培养24h时瘢痕成纤维细胞进入S期的比例明显降低(P<0.01),至72h时已无显著差别.结论 rHu IFN-α.可以抑制瘢痕成纤维细胞的DNA合成及增殖,其机理为rHu IFN-α.对瘢痕成纤维细胞进入有丝分裂S期的抑制,且其效应呈时间依赖性.
-
-
Khan S. The pathology of keloid [J]. SURGERY, 1988.1299. Chvapil M, Koopman C F. Scar formation: physiology and pathological states [J]. Otolaryngol Chn North Am, 1984.265. Kockwell W B. Keloids and hypertrophic scars: a comprehensive review [J]. Plastic and Reconstructive Surgery, 1989(5):827. Granetein K D, Rook A, Flotte T J. Acatralled trial of intralesion reconbinant interferon-r in the trearnent of keloidal scarring [J]. Archives of Dermatology, 1990.1295.doi: 10.1001/archderm.126.10.1295. Duncan M R, Petlish JS. Fleischmajer R:Lymphokine/ rnonokine inhibition of fibroblast proliferation and collagen production:role in progressive sys stemic sclerosis (PSS) [J]. Journal of Investigative Dermatology, 1984, (83):377.doi: 10.1111/1523-1747.ep12264686. Ignotz R A. Transfonning growth factor beta stimulates the expression of fibronectin and collagen and their inoorporation into the extracallular matrix [J]. Journal of Biological Chemistry, 1986.4337. Desmouliere A, Rubbia-Brandt L, Abdin A. A-smooth muscle actin is expressed in a subpopulation of cultured fibroblasts by γ- interferon [J]. Experimental Cell Research, 1992.64.doi: 10.1016/0014-4827(92)90348-C.
计量
- 文章访问数: 340
- HTML全文浏览量: 25
- PDF下载量: 11
下载:
苏公网安备 32100302010246号
